Il1r2 (BC032962) Mouse Tagged ORF Clone

CAT#: MR203789

Il1r2 (Myc-DDK-tagged) - Mouse interleukin 1 receptor, type II (cDNA clone MGC:41170 IMAGE:1282531)

Product Images

Circular map for MR203789

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il1r2
• Synonyms: CD121b; Il1r-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203789 representing BC032962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGAACTCAAGGTCTTTAAGAATACTGAAGCATCTCTGCCTCATGTCTCCTACTTGCAAATCT
  CAGCTCTCTCCACCACCGGGTTACTAGTGTGCCCTGACCTGAAAGAATTCATCTCCAGCAACGCTGATGG
  AAAGATACAGTGGTATAAGGGCGCCATACTCTTGGATAAAGGCAATAAGGAATTTCTGAGTGCAGGAGAC
  CCCACACGCCTATTGATATCCAACACGTCCATGGACGATGCAGGCTATTACAGATGTGTTATGACATTTA
  CCTACAATGGCCAGGAATACAACATCACTAGGAATATTGAACTCCGGGTCAAAGGAACAACCACGGAACC
  CATCCCTGTGATCATTTCTCCCCTGGAGACAATACCAGCATCATTGGGGTCAAGACTGATAGTCCCGTGC
  AAAGTGTTTCTGGGAACTGGTACATCTTCCAACACCATTGTGTGGTGGTTGGCTAACAGCACGTTTATCT
  CGGCTGCTTACCCAAGAGGCCGTGTGACCGAGGGGCTACACCACCAGTACTCAGAGAATGATGAAAACTA
  TGTGGAAGTGTCGCTGATTTTTGATCCAGTCACAAGGGAGGATCTGCATACAGATTTTAAATGTGTTGCC
  TCGAATCCACGGAGTTCTCAGTCACTCCATACCACAGTCAAAGAAGTCTCTTCCACGTTCTCCTGGAGCA
  TTGCGCTGGCACCTCTGTCTCTGATCATCTTGGTTGTGGGGGCAATATGGATGCGCAGACGGTGTAAACG
  CAGGGCTGGAAAGACATATGGACTGACCAAGCTACGGACTGACAACCAGGACTTCCCTTCCAGCCCAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203789 representing BC032962
  Red=Cloning site Green=Tags(s)
  
  MSVELKVFKNTEASLPHVSYLQISALSTTGLLVCPDLKEFISSNADGKIQWYKGAILLDKGNKEFLSAGD
  PTRLLISNTSMDDAGYYRCVMTFTYNGQEYNITRNIELRVKGTTTEPIPVIISPLETIPASLGSRLIVPC
  KVFLGTGTSSNTIVWWLANSTFISAAYPRGRVTEGLHHQYSENDENYVEVSLIFDPVTREDLHTDFKCVA
  SNPRSSQSLHTTVKEVSSTFSWSIALAPLSLIILVVGAIWMRRRCKRRAGKTYGLTKLRTDNQDFPSSPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC032962
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032962, AAH32962
• RefSeq Size: 1096 bp
• RefSeq ORF: 842 bp
• Locus ID: 16178
• Cytogenetics: 1 18.65 cM
• MW: 31.2 kDa
• Gene Summary: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (By similarity).[UniProtKB/Swiss-Prot Function]