Hax1 (NM_011826) Mouse Tagged ORF Clone

CAT#: MR203783

Hax1 (Myc-DDK-tagged) - Mouse HCLS1 associated X-1 (Hax1)

Product Images

Circular map for MR203783

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hax1
• Synonyms: HAX-1; Hs1bp1; HSP1BP-1; mHAX-1s; SIG-111; Silg111
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203783 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTCTTTGATCTTTTCCGAGGCTTTTTCGGCTTTCCTGGACCTCGGAGCCACAGAGATCCTTTTT
  TTGGAGGGATGACTCGAGATGATGACGACGATGATGATGATGATGACGAAGCGGAGGAAGACAGAGGCGC
  GTGGGGTCGAGAGAGCTATGCGTTTGATGGTTCTCAGCCTCCAGAGGAATTCGGTTTCAGCTTCAGCCCC
  AGGGGAGGGATGCGATTCCACGGCAACTTTGGCTTTGATGATCTAGTACGAGATTTTAATAGCATCTTCA
  GCGAGATGGGGGCCTGGACCTTGCCTTCCCACTCTCCTGAACTTCCAGGTCCTGAGTCAGAAACACCTGG
  TGAGAGACTGCGGGAGGGGCAGACACTACGAGACTCAATGCTTAAGTACCCAGATAGTCACCAACCCAGG
  ATCTTTGAGGGGGTCTTGGAGAGTCATGCAAAACCTGAATCCCCAAAACCAGCTCCAGATTGGGGGTCGC
  AGGGACCTTTTCATAGGTTGGATGATACATGGCCTGTGAGTCCCCATTCTAGAGCCAAAGAGGACAAAGA
  TCTTGACTCCCAGGTTTCCCAGGAAGGTCTGGGTCCACTTCTTCAACCCCAGCCCAAATCGTATTTCAAA
  AGTATCTCTGTGACCAAGATCACTAAGCCAGATGGGACAGTGGAGGAACGCCGCACTGTGGTAGACAGTG
  AGGGCCGGAGGGAGACCACAGTGACCCATCAAGAAGCCCATGACAGTTCCAGAAGTGATCCAGACTCTCA
  GAGATCTTCAGCTTTGGATGATCCCTTTTCCATTCTGGATTTGCTACTAGGACGTTGGTTTCGGTCCCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203783 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVFDLFRGFFGFPGPRSHRDPFFGGMTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSP
  RGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPR
  IFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFK
  SISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011826
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011826.4, NP_035956.1
• RefSeq Size: 1175 bp
• RefSeq ORF: 843 bp
• Locus ID: 23897
• UniProt ID: O35387
• Cytogenetics: 3 F1
• MW: 31.7 kDa
• Gene Summary: Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.[UniProtKB/Swiss-Prot Function]