Cd320 (NM_019421) Mouse Tagged ORF Clone

CAT#: MR203391

Cd320 (Myc-DDK-tagged) - Mouse CD320 antigen (Cd320)

Product Images

Circular map for MR203391

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd320
• Synonyms: 8D6; 425O18-1; D17Ertd716e; NG29; TCblR; VLDL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203391 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGGGCGGAGCTGGGCGGGCAGTGGCCCTGGGCCTGGTGCTGCGGCTCCTCTTTGGTCTCAGAA
  CAGGCCTAGAGGCCGCTCCGGCTCCGGCCCATACCCGGGTCCAAGTCTCCGGCTCTAGAGCTGACTCCTG
  CCCGACAGACACCTTCCAGTGTCTTACCAGTGGCTATTGTGTGCCCCTTTCCTGGCGCTGCGATGGGGAC
  CAGGACTGCTCTGATGGCAGTGACGAGGAAGACTGTAGGATTGAGTCATGTGCTCAGAATGGGCAGTGCC
  AACCACAGTCTGCCCTTCCTTGCTCCTGTGACAACATCAGTGGTTGCTCTGATGTCTCTGACAAGAACCT
  CAACTGCAGCCGCCCACCCTGTCAGGAGAGTGAGCTGCATTGCATACTGGATGATGTCTGTATTCCACAC
  ACGTGGCGCTGTGATGGCCACCCGGACTGTCTTGACTCCAGTGATGAGCTCAGCTGTGACACTGACACAG
  AAATTGATAAGATATTCCAGGAGGAGAATGCCACAACTACAAGGATTTCTACGACCATGGAGAATGAGAC
  CTCTTTCAGGAATGTAACATTTACCTCTGCTGGGGACTCATCCCGAAACCCAAGTGCCTATGGGGTTATT
  GCAGCTGCTGGGGTGTTGAGTGCCATCCTGGTTTCGGCCACCCTTCTCATATTGCTACGCCTCCGAGGTC
  AGGGTTATCTGCCCCCACCAGGGCTGTTGGTGGCTGTGAAGGAGTCGCTGCTGCTGTCAGAAAGGAAAAC
  CTCTCTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203391 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHTRVQVSGSRADSCPTDTFQCLTSGYCVPLSWRCDGD
  QDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNLNCSRPPCQESELHCILDDVCIPH
  TWRCDGHPDCLDSSDELSCDTDTEIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYGVI
  AAAGVLSAILVSATLLILLRLRGQGYLPPPGLLVAVKESLLLSERKTSLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019421
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019421.1, NM_019421.2, NM_019421.3, NP_062294.3
• RefSeq Size: 2209 bp
• RefSeq ORF: 783 bp
• Locus ID: 54219
• UniProt ID: Q9Z1P5
• Cytogenetics: 17 17.98 cM
• MW: 27.7 kDa
• Gene Summary: Receptor for transcobalamin saturated with cobalamin (TCbl). Plays an important role in cobalamin uptake. Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells. Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation. Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10.[UniProtKB/Swiss-Prot Function]