Gzma (NM_010370) Mouse Tagged ORF Clone

CAT#: MR203381

Gzma (Myc-DDK-tagged) - Mouse granzyme A (Gzma)

Product Images

Circular map for MR203381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gzma
• Synonyms: AW494114; Ctla-3; Ctla3; Hf; Hf1; SE1; TSP-1; TSP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203381 representing NM_010370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAACGCCTCTGGTCCCCGGGGGCCATCTCTTGCTACTCTCCTTTTTCTTCTGCTTATTCCTGAAG
  GAGGCTGTGAAAGAATCATTGGAGGAGACACGGTTGTTCCTCACTCAAGACCGTATATGGCTCTACTTAA
  ACTTAGTTCAAATACCATCTGTGCTGGCGCTTTGATTGAAAAGAACTGGGTGTTGACTGCTGCCCACTGT
  AACGTGGGAAAGAGATCTAAGTTCATTCTTGGGGCTCACTCAATCAATAAGGAGCCAGAACAACAGATAT
  TGACTGTTAAGAAAGCATTTCCCTATCCATGCTATGATGAATATACACGTGAGGGGGATCTACAACTTGT
  ACGGCTAAAGAAAAAAGCAACAGTTAACAGAAATGTGGCTATCCTTCACCTACCTAAAAAGGGAGATGAT
  GTGAAACCAGGAACCAGATGCCGAGTAGCAGGATGGGGGAGATTTGGCAATAAGTCAGCTCCCTCTGAAA
  CTCTGAGAGAAGTCAACATCACTGTCATAGACAGAAAAATCTGCAATGATGAAAAACACTATAATTTTCA
  TCCTGTAATTGGACTAAACATGATTTGTGCAGGGGACCTCCGTGGTGGAAAGGACTCCTGCAATGGGGAT
  TCTGGCAGCCCTCTGCTATGTGATGGTATTTTGCGAGGCATCACCTCTTTTGGTGGAGAGAAGTGTGGAG
  ATCGCCGATGGCCTGGTGTCTATACTTTCCTCTCAGATAAACACCTCAATTGGATAAAGAAGATTATGAA
  GGGTTCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203381 representing NM_010370
  Red=Cloning site Green=Tags(s)
  
  MRNASGPRGPSLATLLFLLLIPEGGCERIIGGDTVVPHSRPYMALLKLSSNTICAGALIEKNWVLTAAHC
  NVGKRSKFILGAHSINKEPEQQILTVKKAFPYPCYDEYTREGDLQLVRLKKKATVNRNVAILHLPKKGDD
  VKPGTRCRVAGWGRFGNKSAPSETLREVNITVIDRKICNDEKHYNFHPVIGLNMICAGDLRGGKDSCNGD
  SGSPLLCDGILRGITSFGGEKCGDRRWPGVYTFLSDKHLNWIKKIMKGSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010370
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010370.3
• RefSeq Size: 878 bp
• RefSeq ORF: 783 bp
• Locus ID: 14938
• UniProt ID: P11032
• Cytogenetics: 13 63.93 cM
• MW: 29 kDa
• Gene Summary: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (By similarity).[UniProtKB/Swiss-Prot Function]