Bdh2 (NM_027208) Mouse Tagged ORF Clone

CAT#: MR203075

Bdh2 (Myc-DDK-tagged) - Mouse 3-hydroxybutyrate dehydrogenase, type 2 (Bdh2), transcript variant 2

Product Images

Circular map for MR203075

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bdh2
• Synonyms: 1810026B04Rik; Dhrs6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203075 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGACTGGACGGCAAAGTTATTGTCCTGACAGCTGCCGCTCAAGGGATTGGACGGGCATCCGCAT
  TAGCTTTTGCAAGAGAAGGAGCCAAAGTCATAGCCACAGATATCAACGAGTCCAAACTCCAGGAGCTGGA
  AAGTTACCGAGGTATTCAAACTCGAGTCCTTGATGTCACAAAGAAGAGACAGATTGATCAGTTTGCCTCA
  GAAATCGAGAGAATTGATGTTCTCTTTAATGTTGCTGGTTTTGTCCACCACGGAACCATCCTGGATTGCG
  AGGAAAAAGACTGGGACTTCTCAATGAATCTCAACGTCCGCAGCATGTTCCTGATGATCAAAGCATTCCT
  TCCCAAAATGCTTGCTCAGAAGTCAGGCAACATTATCAACATGTCGTCTGTGGCCTCCAGCATCAAAGGG
  GTGGAGAACAGATGTGTGTACAGTGCTACCAAGGCGGCTGTGATCGGTCTCACCAAGTCCGTGGCTGCAG
  ACTTCATCCAACAGGGCATCAGATGCAACTGTGTGTGTCCAGGAACGGTTGACACCCCATCTCTGCAAGA
  AAGAATACAAGCCAGAGACAATCCCAAAGAGGCACTGAAAACTTTCCTAAACAGACAAAAGACGGGAAGG
  TTTGCGTCGGCCGAAGAGGTCGCTCTGCTCTGCGTATACCTGGCCTCGGACGAGTCAGCCTATGTAACTG
  GCAACCCTGTCATCATTGATGGGGGCTGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203075 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRLDGKVIVLTAAAQGIGRASALAFAREGAKVIATDINESKLQELESYRGIQTRVLDVTKKRQIDQFAS
  EIERIDVLFNVAGFVHHGTILDCEEKDWDFSMNLNVRSMFLMIKAFLPKMLAQKSGNIINMSSVASSIKG
  VENRCVYSATKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARDNPKEALKTFLNRQKTGR
  FASAEEVALLCVYLASDESAYVTGNPVIIDGGWSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027208
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027208.2
• RefSeq Size: 1099 bp
• RefSeq ORF: 738 bp
• Locus ID: 69772
• UniProt ID: Q8JZV9
• Cytogenetics: 3 G3
• MW: 26.8 kDa
• Gene Summary: Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis (PubMed:20550936, PubMed:24863067). Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (PubMed:24863067). Also acts as a 3-hydroxybutyrate dehydrogenase (By similarity).[UniProtKB/Swiss-Prot Function]