Fgf8 (NM_001166361) Mouse Tagged ORF Clone

CAT#: MR203044

Fgf8 (Myc-DDK-tagged) - Mouse fibroblast growth factor 8 (Fgf8), transcript variant 2

Product Images

Circular map for MR203044

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgf8
• Synonyms: Aigf; Fgf-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203044 representing NM_001166361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCCCCCGCTCCGCGCTGAGCTGCCTGCTGTTGCACTTGCTGGTTCTCTGCCTCCAAGCCCAGG
  AAGGCCCGGGCGGGGGGCCTGCGCTGGGCAGGGAGCCCACTTCCCTGCTCCGAGCTGGCCGGGAGCCCCA
  GGGTGTTTCCCAACAGGTAACTGTTCAGTCCTCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTG
  GTGACGGATCAGCTCAGCCGCCGCCTCATCCGGACCTACCAGCTCTACAGCCGCACCAGCGGGAAGCACG
  TGCAGGTCCTGGCCAACAAGCGCATCAACGCCATGGCAGAAGACGGAGACCCCTTCGCGAAGCTCATTGT
  GGAGACCGATACTTTTGGAAGCAGAGTCCGAGTTCGCGGCGCAGAGACAGGTCTCTACATCTGCATGAAC
  AAGAAGGGGAAGCTAATTGCCAAGAGCAACGGCAAAGGCAAGGACTGCGTATTCACAGAGATCGTGCTGG
  AGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTTACCCGCAAGGGCCG
  GCCCCGCAAGGGCTCCAAGACGCGCCAGCATCAGCGCGAGGTGCACTTCATGAAGCGCCTGCCGCGGGGC
  CACCACACCACCGAGCAGAGCCTGCGCTTCGAGTTCCTCAACTACCCGCCCTTCACGCGCAGCCTGCGCG
  GCAGCCAGAGGACTTGGGCCCCGGAGCCCCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203044 representing NM_001166361
  Red=Cloning site Green=Tags(s)
  
  MGSPRSALSCLLLHLLVLCLQAQEGPGGGPALGREPTSLLRAGREPQGVSQQVTVQSSPNFTQHVREQSL
  VTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMN
  KKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRG
  HHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166361
• ORF Size: 732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166361.1, NP_001159833.1
• RefSeq Size: 1188 bp
• RefSeq ORF: 735 bp
• Locus ID: 14179
• Cytogenetics: 19 38.75 cM
• MW: 28 kDa
• Gene Summary: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells (By similarity). Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis (PubMed:7884899).[UniProtKB/Swiss-Prot Function]