Schip1 (BC060529) Mouse Tagged ORF Clone

CAT#: MR203032

Schip1 (Myc-DDK-tagged) - Mouse schwannomin interacting protein 1 (cDNA clone MGC:69575 IMAGE:5693616)

Product Images

Circular map for MR203032

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Schip1
• Synonyms: Nf2ip, SCHIP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203032 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACATCAGGAGAACTGCTCGTACCAGGCACAGAAGAATGAGAGAGAGTCTATCAGACAGAAGTTGG
  CACTCGGAAGCTTCTTTGACGATGGCCCAGGAATCTATACCAGCTGCAGCAAAAGTGGGAAGCCAAGCCT
  TTCTGCAAGACTACAGAGCGGGATGAACCTCCAGATATGCTTTGTCAATGACAGCGGCAGTGACAAGGAC
  AGCGATGCAGATGACAGTAAGACGGAAACCAGCTTGGACACGCCCTTGTCCCCCATGAGCAAGCAGAGTT
  CTTCCTATTCGGATAGAGACACAACTGAGGAGGAGTCTGAATCCCTGGATGACATGGACTTCCTCACAAG
  GCAAAAGAAGCTACAAGCTGAAGCCAAAATGGCTCTGGCCATGGCCAAACCAATGGCCAAAATGCAAGTA
  GAAGTGGAAAAACAGAACAGGAAAAAGTCTCCCGTCGCTGATCTTCTCCCACACATGCCTCACATAAGCG
  AATGTTTGATGAAAAGAAGCTTAAAGCCCACCGACCTGAGAGACATGACTATCGGGCAGCTACAAGTGAT
  CGTCAATGACCTCCACTCCCAGATTGAAAGTTTGAATGAAGAGTTGGTCCAGCTGCTCCTTATTCGAGAT
  GAGCTGCACACAGAACAAGATGCCATGCTGGTGGACATTGAAGACTTGACTAGACACGCTGAGAGTCAGC
  AGAAGCACATGGCTGAGAAAATGCCCGCGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203032 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHQENCSYQAQKNERESIRQKLALGSFFDDGPGIYTSCSKSGKPSLSARLQSGMNLQICFVNDSGSDKD
  SDADDSKTETSLDTPLSPMSKQSSSYSDRDTTEEESESLDDMDFLTRQKKLQAEAKMALAMAKPMAKMQV
  EVEKQNRKKSPVADLLPHMPHISECLMKRSLKPTDLRDMTIGQLQVIVNDLHSQIESLNEELVQLLLIRD
  ELHTEQDAMLVDIEDLTRHAESQQKHMAEKMPAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060529
• ORF Size: 732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060529, AAH60529
• RefSeq Size: 1569 bp
• RefSeq ORF: 734 bp
• Locus ID: 30953
• Cytogenetics: 3 E1
• MW: 27.5 kDa