Fam3c (NM_138587) Mouse Tagged ORF Clone

CAT#: MR202685

Fam3c (Myc-DDK-tagged) - Mouse family with sequence similarity 3, member C (Fam3c)

Product Images

Circular map for MR202685

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fam3c
• Synonyms: D6Wsu176e; Ilei
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202685 representing NM_138587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTAGCAGGAGCTGCAAAGTTGGTAGTGGCCGTGGCAGTATTCTTACTGACCTTCTATGTTATTT
  CTCAAGTATTTGAAATTAAAATGGATGCAAGTTTAGGAAATCTATTTGCTCGATCCGCGCTGGACTCAGC
  CATTCGTTCTACGAAACCTCCGAGGTACAAGTGTGGGATCTCAAAGGCGTGCCCAGAGAAGCATTTTGCT
  TTTAAGATGGCTAGTGGAGCAGCCAATGTCGTGGGACCCAAGATCTGCCTGGAGGACAATGTTTTGATGA
  GTGGTGTGAAGAATAATGTCGGAAGAGGAATCAATATTGCCTTGGTAAATGGGAAAACAGGGGAAGTAAT
  AGACACCAAATTTTTTGACATGTGGGGAGGAGATGTGGCACCATTCATTGAGTTTTTGAAGACCATACAA
  GACGGAACAGTAGTGCTAATGGCTACATACGATGATGGAGCAACCAAACTCACGGATGAGGCACGGCGGC
  TCATTGCTGAACTGGGCAGCACTTCGATCACCAGTCTTGGTTTCCGAGATAACTGGGTCTTCTGTGGTGG
  GAAGGGCATTAAGACAAAGAGTCCCTTTGAACAGCACATAAAGAACAATAAGGAAACGAACAAGTACGAG
  GGATGGCCTGAGGTGGTGGAGATGGAAGGATGTATCCCCCAGAAGCAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202685 representing NM_138587
  Red=Cloning site Green=Tags(s)
  
  MRVAGAAKLVVAVAVFLLTFYVISQVFEIKMDASLGNLFARSALDSAIRSTKPPRYKCGISKACPEKHFA
  FKMASGAANVVGPKICLEDNVLMSGVKNNVGRGINIALVNGKTGEVIDTKFFDMWGGDVAPFIEFLKTIQ
  DGTVVLMATYDDGATKLTDEARRLIAELGSTSITSLGFRDNWVFCGGKGIKTKSPFEQHIKNNKETNKYE
  GWPEVVEMEGCIPQKQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138587
• ORF Size: 681 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138587.4, NP_613053.3
• RefSeq Size: 2739 bp
• RefSeq ORF: 684 bp
• Locus ID: 27999
• UniProt ID: Q91VU0
• Cytogenetics: 6 9.24 cM
• MW: 25.2 kDa
• Gene Summary: May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.[UniProtKB/Swiss-Prot Function]