Thoc7 (NM_025435) Mouse Tagged ORF Clone

CAT#: MR202136

Thoc7 (Myc-DDK-tagged) - Mouse THO complex 7 homolog (Drosophila) (Thoc7), transcript variant 1

Product Images

Circular map for MR202136

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thoc7
• Synonyms: Nif3l1bp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202136 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCCGTGACTGACGACGAAGTTATACGGAAACGTCTTCTGATTGATGGAGATGGTGCTGGGGATG
  ATCGGAGAATTAATCTCCTCGTGAAAAGCTTCATTAAATGGTGCAACTCTGGATCCCAAGAGGAAGGGTA
  TAGCCAGTACCAACGCATGCTGAGCACTCTGTCCCAGTGTGAATTTTCAATGGGCAAAACATTGCTGGTA
  TATGATATGAATCTCAGAGAAATGGAAAATTATGAAAAAATATACAAAGAAATAGAATGTAGTATTGCTG
  GAGCACATGAAAAAATTGCTGAGTGTAAAAAGCAGATTCTTCAAGCAAAACGAATACGAAAAAATCGACA
  AGAATATGACGCTTTGGCCAAAATGATCCAGCATCACCCAGATAGGCATGAGACACTGAAGGAGCTAGAG
  GCTCTGGGCAAAGAATTAGAGCATCTCTCACATATTAAAGAAAGTGTTGAAGATAAGCTGGAATTGAGAC
  GGAAACAATTTCACGTTCTTCTTAGTACCATCCATGAACTTCAACAGACATTGGAGAATGATGACAAGCT
  GTCAGAGGTGGATGAAGCTCAAGAAAGCACCATGGAAGCAGACCCTAAGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202136 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFIKWCNSGSQEEGYSQYQRMLSTLSQCEFSMGKTLLV
  YDMNLREMENYEKIYKEIECSIAGAHEKIAECKKQILQAKRIRKNRQEYDALAKMIQHHPDRHETLKELE
  ALGKELEHLSHIKESVEDKLELRRKQFHVLLSTIHELQQTLENDDKLSEVDEAQESTMEADPKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025435
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025435.1, NM_025435.2, NM_025435.3, NP_079711.1
• RefSeq Size: 991 bp
• RefSeq ORF: 615 bp
• Locus ID: 66231
• UniProt ID: Q7TMY4
• Cytogenetics: 14 A1
• MW: 23.7 kDa
• Gene Summary: Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.[UniProtKB/Swiss-Prot Function]