C8g (NM_027062) Mouse Tagged ORF Clone
CAT#: MR202091
- TrueORF®
C8g (Myc-DDK-tagged) - Mouse complement component 8, gamma polypeptide (C8g)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_027062" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | C8g |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202091 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCTCCAGGGGCTGTGTTGTTCTTCACCCTGCTCTTGACAGCCAGTTCGCTGGGCCAAAGGACTC GAAAGCCTATTGGGTCTACCTCCCCCATCAGCACTATCCAGGCCCAGGTCAATTTCAGTGCTCAGAAGTT TGCAGGGACATGGCTCCTGGTGGCTGTGGGCTCTTCGTGCCGCTTTCTTCAGGAGCAGGGACACCGAGCT GAGGCCACCACATTGCACGCAGCTCCCCAGGGTGCAGCTATGGCTGTCAGCACCTTCCGAAAGCTAGATG GGATATGTTGGCAGGTACGCCAACTCTTTGAGAACACAGGGGTCCCTGGCCGCTTCTTGTTCCAAGTCTC ACGGGCCCGTGGACCAGTGCATATGGTGGTTGCTGAGACTGACTATCAGAGCTTTGCCATCCTGTACCTG GAACAGGGAAGGAAGCTGTCTGTGAAACTATACGTCCGCTCACTGCCAGTGAATGACTCTGTCCTGGATG TGTTTGAGCGGCGAGTCAGGGAAGCCAACCTGACAGAAGATCAGATTCTTTTCTTTCCCAAGTATGGTTT CTGCGAGACTGCGGACCAATTACACATCCTGAACGAGGTGCCGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202091 protein sequence
Red=Cloning site Green=Tags(s) MLSPGAVLFFTLLLTASSLGQRTRKPIGSTSPISTIQAQVNFSAQKFAGTWLLVAVGSSCRFLQEQGHRA EATTLHAAPQGAAMAVSTFRKLDGICWQVRQLFENTGVPGRFLFQVSRARGPVHMVVAETDYQSFAILYL EQGRKLSVKLYVRSLPVNDSVLDVFERRVREANLTEDQILFFPKYGFCETADQLHILNEVPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027062 |
ORF Size | 609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027062.2 |
RefSeq Size | 1044 bp |
RefSeq ORF | 609 bp |
Locus ID | 69379 |
UniProt ID | Q8VCG4 |
Cytogenetics | 2 17.31 cM |
MW | 22.5 kDa |
Gene Summary | This gene encodes a secreted protein that is a core component of the complement 8 (C8) complex. C8 is part of the membrane attack complex which participates in the innate immune response against bacterial pathogens. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203121 | C8g (untagged) - Mouse complement component 8, gamma polypeptide (C8g), (10ug) |
USD 300.00 |
|
MG202091 | C8g (tGFP-tagged) - Mouse complement component 8, gamma subunit (C8g) |
USD 500.00 |
|
MR202091L3 | Lenti ORF clone of C8g (Myc-DDK-tagged) - Mouse complement component 8, gamma polypeptide (C8g) |
USD 600.00 |
|
MR202091L4 | Lenti ORF clone of C8g (mGFP-tagged) - Mouse complement component 8, gamma polypeptide (C8g) |
USD 600.00 |
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