Sar1b (NM_025535) Mouse Tagged ORF Clone

CAT#: MR201981

Sar1b (Myc-DDK-tagged) - Mouse SAR1 gene homolog B (S. cerevisiae) (Sar1b)

Product Images

Circular map for MR201981

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sar1b
• Synonyms: 2310075M17Rik; 2900019I22Rik; CMRD; Sara1b; Sara2; Sarb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201981 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCATATTTGACTGGATTTACAGTGGTTTCAGCAGTGTGCTGCAGTTTCTAGGATTGTATAAAA
  AGTCTGGTAAACTGGTATTCCTTGGATTGGATAATGCCGGGAAAACAACTTTGCTACACATGCTGAAAGA
  TGACAGGCTCGGGCAGCACGTCCCAACGCTACATCCCACTTCAGAAGAGCTTACTATTGCTGGCATGACG
  TTTACAACTTTTGATCTGGGTGGGCACGTGCAAGCCCGAAGAGTATGGAAAAACTACCTTCCTGCTATCA
  ATGGCATTGTATTTCTTGTGGATTGTGCAGACCATGAAAGGCTGTTAGAATCAAAAGAAGAACTTGATTC
  ACTAATGACAGATGAAACCATTGCCAACGTGCCTATTCTAATTCTTGGAAATAAGATCGACAGACCTGAA
  GCCATCAGTGAAGAGAGGTTACGAGAGATGTTTGGTTTATATGGTCAGACAACAGGAAAGGGTAGTGTGT
  CACTGAAGGAGCTGAACGCCCGGCCTCTGGAAGTGTTCATGTGCAGTGTGCTAAAAAGGCAGGGCTATGG
  AGAAGGCTTCCGCTGGATGGCGCAGTACATCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201981 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFIFDWIYSGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMT
  FTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPE
  AISEERLREMFGLYGQTTGKGSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025535
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025535.2
• RefSeq Size: 1253 bp
• RefSeq ORF: 597 bp
• Locus ID: 66397
• UniProt ID: Q9CQC9
• Cytogenetics: 11 B1.3
• MW: 22.4 kDa
• Gene Summary: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.[UniProtKB/Swiss-Prot Function]