Gm2a (NM_010299) Mouse Tagged ORF Clone

CAT#: MR201862

Gm2a (Myc-DDK-tagged) - Mouse GM2 ganglioside activator protein (Gm2a)

Product Images

Circular map for MR201862

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gm2a
• Synonyms: AA408702; AW215435; GM2-AP; SAP-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201862 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCGTCTACCGCTGCTGCTCCTGCTGGGCTTGCTGCTCGCAGGCTCCGTCGCCCCTGCGCGCCTCG
  TCCCGAAGCGCCTTTCCCAACTTGGTGGCTTCTCCTGGGATAACTGTGATGAAGGAAAGGACCCTGCAGT
  GATCAAAAGCCTCACGATCCAACCTGACCCCATTGTGGTTCCTGGAGATGTAGTCGTCAGCCTTGAGGGC
  AAGACCAGCGTTCCCCTCACTGCTCCTCAGAAGGTGGAGCTCACCGTGGAGAAGGAAGTGGCTGGCTTCT
  GGGTCAAGATTCCTTGTGTAGAACAGCTAGGCAGCTGTAGCTACGAGAACATCTGTGACCTGATAGACGA
  ATACATTCCTCCTGGAGAGAGCTGCCCAGAGCCCCTGCACACCTACGGGCTGCCCTGCCACTGTCCCTTC
  AAGGAAGGTACCTACTCACTACCCACGAGCAACTTCACAGTGCCTGACCTAGAGCTACCGAGCTGGCTGA
  GCACAGGCAACTACCGCATCCAGAGTATCTTGAGCAGTGGTGGGAAGCGCCTGGGCTGCATCAAGATTGC
  TGCCTCTCTCAAGGGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201862 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHRLPLLLLLGLLLAGSVAPARLVPKRLSQLGGFSWDNCDEGKDPAVIKSLTIQPDPIVVPGDVVVSLEG
  KTSVPLTAPQKVELTVEKEVAGFWVKIPCVEQLGSCSYENICDLIDEYIPPGESCPEPLHTYGLPCHCPF
  KEGTYSLPTSNFTVPDLELPSWLSTGNYRIQSILSSGGKRLGCIKIAASLKGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010299
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4257 bp
• RefSeq ORF: 582 bp
• Locus ID: 14667
• UniProt ID: Q60648
• Cytogenetics: 11 32.13 cM
• MW: 20.8 kDa
• Gene Summary: Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity.[UniProtKB/Swiss-Prot Function]