Nudt4 (NM_027722) Mouse Tagged ORF Clone
CAT#: MR201593
- TrueORF®
Nudt4 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 4 (Nudt4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_027722" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Nudt4 |
Synonyms | 4933436C10Rik; DIPP2; DIPP2alpha; DIPP2beta; HDCMB47P |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201593 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTCAAGCCCAACCAGACGCGGACGTACGACCGCGAGGGCTTCAAGAAGAGGGCGGCCTGCCTGT GCTTCCGCAGCGAGCAGGAGGACGAGGTGCTGCTGGTGAGTAGCAGTCGGTACCCAGACCAATGGATTGT CCCAGGAGGAGGGATGGAGCCGGAGGAGGAGCCTGGCGGTGCTGCTGTGAGGGAAGTGTATGAAGAGGCT GGAGTCAAAGGAAAACTAGGCAGACTCCTGGGAATATTTGAGAATCAAGACCGGAAGCACAGAACATATG TTTATGTTCTGACCGTCACTGAAATATTGGAAGACTGGGAAGACTCCGTGAATATAGGAAGGAAGAGAGA GTGGTTCAAAGTAGAAGATGCAATCAAGGTTCTTCAGTGTCATAAGCCTGTCCACGCAGAGTATCTGGAA AAACTAAAGCTGGGCTGTTCTCCGACCAATGGAAATTCCTCGGTCCCTTCCCTCCCAGATAACAATGCCT TGTTTGTGACTGCTGCGCCGCCCTCTGGGGTGCCATCCAGTATAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201593 protein sequence
Red=Cloning site Green=Tags(s) MKFKPNQTRTYDREGFKKRAACLCFRSEQEDEVLLVSSSRYPDQWIVPGGGMEPEEEPGGAAVREVYEEA GVKGKLGRLLGIFENQDRKHRTYVYVLTVTEILEDWEDSVNIGRKREWFKVEDAIKVLQCHKPVHAEYLE KLKLGCSPTNGNSSVPSLPDNNALFVTAAPPSGVPSSIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027722 |
ORF Size | 540 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027722.1, NM_027722.2, NM_027722.3, NM_027722.4, NP_081998.2 |
RefSeq Size | 3231 bp |
RefSeq ORF | 540 bp |
Locus ID | 71207 |
UniProt ID | Q8R2U6 |
Cytogenetics | 10 C2 |
MW | 20.2 kDa |
Gene Summary | Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphate Ap6A, but not Ap5A. The major reaction products are ADP and p4a from Ap6A. Also able to hydrolyze 5-phosphoribose 1-diphosphate (By similarity). Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201451 | Nudt4 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 4 (Nudt4), (10ug) |
USD 300.00 |
|
MG201593 | Nudt4 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 4 (Nudt4) |
USD 500.00 |
|
MR201593L3 | Lenti ORF clone of Nudt4 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 4 (Nudt4) |
USD 600.00 |
|
MR201593L4 | Lenti ORF clone of Nudt4 (mGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 4 (Nudt4) |
USD 600.00 |
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