Mobp (NM_001039365) Mouse Tagged ORF Clone

CAT#: MR201417

Mobp (Myc-DDK-tagged) - Mouse myelin-associated oligodendrocytic basic protein (Mobp), transcript variant 3

Product Images

Circular map for MR201417

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mobp
• Synonyms: AI835053; AI839848; MOBP69; MOBP73; MOBP81; MOBP155; MOBP170; R74645
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201417 representing NM_001039365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGAAAATGGCCAAGGAGGGCCCCAGGCTCTCCAAGAACCAGAAGTTCTCCGAGCACTTCAGCA
  TCCACTGCTGCCCACCCTTCACCTTCCTCAACTCCAAGCGTGAGATCGTGGACCGCAAGTACAGCATCTG
  CAAGAGCGGTTGCTTTTACCAGAAGAAGGAGGAGGACTGGATCTGCTGTGCCTGCCAGAAGACTAGCCGC
  CGTGCCACGTCCCCTCAGAGGCCCAAGCACCAGCCAGCTGCGTCCCCAGTGGTGGTCAGAGCGCCGCCAG
  CCAAGCCAAAGTCCCCTCTGATGCCAGCCAAGCCAAGGTCCCCACCGAGGCCAGCCAAGCCAAGGTCCCC
  TTCAAGGACTGAGCGCCAGCCGCGTCCCCGCCCAGAGGTCCGACCACCACCAGCCAAGCAGAAGCCCCCT
  CAGAAGTCTAAGCAGCCAGCACGCAGCAGCCCCCTCAGAGGGCCAGGCACCAGCCGCGGGGGGTCTCCCA
  CCAGAGCTCCTAGGTTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201417 representing NM_001039365
  Red=Cloning site Green=Tags(s)
  
  MSQKMAKEGPRLSKNQKFSEHFSIHCCPPFTFLNSKREIVDRKYSICKSGCFYQKKEEDWICCACQKTSR
  RATSPQRPKHQPAASPVVVRAPPAKPKSPLMPAKPRSPPRPAKPRSPSRTERQPRPRPEVRPPPAKQKPP
  QKSKQPARSSPLRGPGTSRGGSPTRAPRFW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039365
• ORF Size: 510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039365.3
• RefSeq Size: 1499 bp
• RefSeq ORF: 513 bp
• Locus ID: 17433
• UniProt ID: Q9D2P8
• Cytogenetics: 9 71.41 cM
• MW: 19.2 kDa
• Gene Summary: May play a role in compacting or stabilizing the myelin sheath possibly by binding the negatively charged acidic phospholipids of the cytoplasmic membrane.[UniProtKB/Swiss-Prot Function]