Dnajc15 (NM_025384) Mouse Tagged ORF Clone

CAT#: MR201048

Dnajc15 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 15 (Dnajc15)

Product Images

Circular map for MR201048

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnajc15
• Synonyms: 1110003P16Rik; Dnajd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201048 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCGGTGGCGGCGTGACCTCCAGAGAGGGGCTGCGCTACGCCGAATACCTGCCTCCTTCTGCCC
  AAAGGTCGGACGCCGACATCGACCACACAGCGGGGAGAAGGTTGCTAGCTGTAGGACTAGGTGTTGCAGC
  TGTTGCATTTGCAGGTCGCTATGCATTTCAGATCTGGAAACCTCTAGAACAAGTAATCACGGCAACAGCA
  AGGAAGATTTCCTCTCCAAGCTTTTCATCCTACTATAAAGGAGGATTCGAGCAGAAAATGAGTAAGCGAG
  AGGCTAGTCTTATTTTAGGTGTAAGCCCATCTGCTGGCAAGGCCAAGATTAGAACAGCACACAAGAGAAT
  TATGATTTTAAACCATCCAGACAAAGGTGGATCTCCTTACTTAGCATCCAAAATAAATGAAGCAAAAGAT
  TTGCTCGAAGCATCCAGCAAAGCTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201048 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATGGGVTSREGLRYAEYLPPSAQRSDADIDHTAGRRLLAVGLGVAAVAFAGRYAFQIWKPLEQVITATA
  RKISSPSFSSYYKGGFEQKMSKREASLILGVSPSAGKAKIRTAHKRIMILNHPDKGGSPYLASKINEAKD
  LLEASSKAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025384
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025384.3, NP_079660.1
• RefSeq Size: 671 bp
• RefSeq ORF: 450 bp
• Locus ID: 66148
• UniProt ID: Q78YY6
• Cytogenetics: 14 D3
• MW: 16 kDa
• Gene Summary: Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9 (By similarity). Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP.[UniProtKB/Swiss-Prot Function]