Crb3 (NM_177638) Mouse Tagged ORF Clone

CAT#: MR200450

Crb3 (Myc-DDK-tagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3)

Product Images

Circular map for MR200450

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crb3
• Synonyms: 5730439B18; AU015179; AU041752
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200450 representing NM_177638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCAGGCCTGGGGGTGCTCTTGGCGTTTGGTTTGCCGATGCTGCCCTCCGGCTGGAGCCTAA
  CAGCACCGGACCCTTTCACAAATAGCACAACTCAACCCCCAGGTGATGAGTCCAACGGGGGCCTGTCTTC
  AGGGGCTATTGTAGCCATCACTGTGGTCTTTTCCATTCTGGGAGTCCTCCTTATAGCAGTGGGACTGTTT
  CTGCTCATGCGAAAACTTCGGGAAAAGCGGCAGACAGAGGGCACCTACCGGCCCAGCAGTGAGGAGCAGG
  TGGGTGCCCGTGCTCCACCACCCCCCAACCTCAAGCTGCCCCCGGAGGAGCGGCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200450 representing NM_177638
  Red=Cloning site Green=Tags(s)
  
  MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLF
  LLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177638
• ORF Size: 339 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177638.5
• RefSeq Size: 1160 bp
• RefSeq ORF: 342 bp
• Locus ID: 224912
• UniProt ID: Q8QZT4
• Cytogenetics: 17 D
• MW: 12.4 kDa
• Gene Summary: Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions (By similarity).[UniProtKB/Swiss-Prot Function]