Slc9b2 (NM_178877) Mouse Tagged ORF Clone

CAT#: MG208700

Slc9b2 (tGFP-tagged) - Mouse expressed sequence C80638 (C80638)

Product Images

Circular map for MG208700

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc9b2
• Synonyms: C80638; nha-oc; NHA2; nhaoc; NHE10; Nhedc2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208700 representing NM_178877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGAAGATAAGACAGCTGAATGTCAGCATTCAAAGCCACCTACGGGGATCACACACGAGGCTC
  CTCCACATCACGAACTACAGGAAGAGAGAGTCATGAGCCTCAGAGGTACAGACAGAAGTGAACCGACAGA
  AGGCAGTAACCTGCTGACCAGCGGTGAAAAAAAGCCACAGGACTCACCAACAGAACCCAATGGCCTGCAG
  AGTCTGAGGCGATTCCTGGCCTGCCCTCCACGAGGCTGCCTGGCAAGAGTGATAACGAACGGTACCATGG
  TTGTTCTTCTGTGGGCCATGGTTTGGTCAGTTACCGGCCCTGAATGTCTTCCTGGAGGAAATCTGTTTGG
  AATTATCATTCTGTTCTATTGTTCCATCACCGGAGGTAAACTTTTTGGACTCATTAAGTTTCCAACATTG
  CCTCCTCTGCCTCCTCTTCTTGGCATGCTGCTAGCTGGGTTTCTCTTGAGGAATATCCCAGTCATCAATG
  ATAGCGTCCGGATCCAACACAAGTGGTCGTCATCTTTGAGAAGCATAGCCCTTTCTGTCATTCTGGTTCG
  TGCTGGCCTTGGTCTAGATTCAAAGGCCCTGAGGAAGCTGAAGGGTGTGTGTGTGCGACTGGCCATGGGT
  CCCTGCATCGTGGAGGCGTGTGCTTCTGCGATTCTCTCACACTTCCTGATGGGGTTGCCATGGCAATGGG
  GGTTCATCCTGGGTTTTGTCGTAGGTGCCGTGTCCCCAGCTGTCGTGGTGCCCTCCATGCTCCTTTTGCA
  GGAAGGAGGCTACGGTGTTGGAAAAGGTATCCCAACCTTACTCATGGCCGCCGGCAGCTTCGATGACATC
  CTGGCCATCACTGGCTTCAACACGTGCTTAGGCGTGGCCTTTTCCACAGGATCTACAGTTTTTAACATCT
  TCAGAGGCATCTTGGAGGTGGTAATTGGTGTGGCAGCTGGATCTTTTCTTGGGTTTTTTATCCAGTACTT
  CCCGAGCAGGGACCAGGACAACCTCGTGTGGAAGCGAGCCTTTCTGGTTCTGGGTTTTGCTGTGCTCGCT
  GTGTTCAGCAGTGTGTATTTTAGCTTCCCGGGGTCTGGAGGACTCTGCACGTTGGTCATGGCTTTCCTAG
  CAGGCATGAGGTGGACTGACAAGAAGTCAGAGGTAGAAAAGGTCATTGCAGTTACCTGGGACGTTTTCCA
  GCCTCTTCTTTTTGGCCTGATTGGAGCAGAGGTTTCCATTGTGTCTCTCAGAGCAGAAACGGTTGGCCTT
  TGTGTTGCAACCCTCAGCATCGCAGTGCTTATACGAATTCTGACTACATTCCTGATGGTGTGTTTCGCTG
  GCTTTAACATAAAGGAAAAGATATTTATTTCTTTTGCCTGGCTTCCAAAGGCCACGGTCCAGGCTGCCAT
  TGGCTCTGTGGCTCTGGACACGGCAAGATCCCACGGAGAGAAGCAGCTGGAAGACTATGGGATGGATGTG
  CTGACGGTGGCATTTTTGGCCATCCTCATTACAGCACCAATTGGAAGCCTACTGATTGGTTTGCTGGGTC
  CCAGGGTTCTTCAGAAATCTGAACATCGAACCGAAGAGGAGGTTCAAGGAGAGACTTCTGCACACATTCA
  GAGGAAGCCTGAGGATTCCATTACGGAAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208700 representing NM_178877
  Red=Cloning site Green=Tags(s)
  
  MEDEDKTAECQHSKPPTGITHEAPPHHELQEERVMSLRGTDRSEPTEGSNLLTSGEKKPQDSPTEPNGLQ
  SLRRFLACPPRGCLARVITNGTMVVLLWAMVWSVTGPECLPGGNLFGIIILFYCSITGGKLFGLIKFPTL
  PPLPPLLGMLLAGFLLRNIPVINDSVRIQHKWSSSLRSIALSVILVRAGLGLDSKALRKLKGVCVRLAMG
  PCIVEACASAILSHFLMGLPWQWGFILGFVVGAVSPAVVVPSMLLLQEGGYGVGKGIPTLLMAAGSFDDI
  LAITGFNTCLGVAFSTGSTVFNIFRGILEVVIGVAAGSFLGFFIQYFPSRDQDNLVWKRAFLVLGFAVLA
  VFSSVYFSFPGSGGLCTLVMAFLAGMRWTDKKSEVEKVIAVTWDVFQPLLFGLIGAEVSIVSLRAETVGL
  CVATLSIAVLIRILTTFLMVCFAGFNIKEKIFISFAWLPKATVQAAIGSVALDTARSHGEKQLEDYGMDV
  LTVAFLAILITAPIGSLLIGLLGPRVLQKSEHRTEEEVQGETSAHIQRKPEDSITEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178877
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178877.6, NP_849208.4
• RefSeq Size: 2243 bp
• RefSeq ORF: 1644 bp
• Locus ID: 97086
• UniProt ID: Q5BKR2
• Cytogenetics: 3 G3
• Gene Summary: Na(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane (PubMed:18508966, PubMed:17988971). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (PubMed:23720317). Involved in sperm motility and fertility (PubMed:27010853). It is controversial whether SLC9B2 plays a role in osteoclast differentiation (PubMed:17988971) or not (PubMed:20441802, PubMed:22985540).[UniProtKB/Swiss-Prot Function]