Slc9b2 (NM_178877) Mouse Untagged Clone

CAT#: MC218862

Slc9b2 (untagged) - Mouse Na+/H+ exchanger domain containing 2 (Nhedc2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc9b2
• Synonyms: C80638; nha-oc; NHA2; nhaoc; NHE10; Nhedc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218862 representing NM_178877
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGAAGATAAGACAGCTGAATGTCAGCATTCAAAGCCACCTACGGGGATCACACACGAGGCTC
  CTCCACATCACGAACTACAGGAAGAGAGAGTCATGAGCCTCAGAGGTACAGACAGAAGTGAACCGACAGA
  AGGCAGTAACCTGCTGACCAGCGGTGAAAAAAAGCCACAGGACTCACCAACAGAACCCAATGGCCTGCAG
  AGTCTGAGGCGATTCCTGGCCTGCCCTCCACGAGGCTGCCTGGCAAGAGTGATAACGAACGGTACCATGG
  TTGTTCTTCTGTGGGCCATGGTTTGGTCAGTTACCGGCCCTGAATGTCTTCCTGGAGGAAATCTGTTTGG
  AATTATCATTCTGTTCTATTGTTCCATCACCGGAGGTAAACTTTTTGGACTCATTAAGTTTCCAACATTG
  CCTCCTCTGCCTCCTCTTCTTGGCATGCTGCTAGCTGGGTTTCTCTTGAGGAATATCCCAGTCATCAATG
  ATAGCGTCCGGATCCAACACAAGTGGTCGTCATCTTTGAGAAGCATAGCCCTTTCTGTCATTCTGGTTCG
  TGCTGGCCTTGGTCTAGATTCAAAGGCCCTGAGGAAGCTGAAGGGTGTGTGTGTGCGACTGGCCATGGGT
  CCCTGCATCGTGGAGGCGTGTGCTTCTGCGATTCTCTCACACTTCCTGATGGGGTTGCCATGGCAATGGG
  GGTTCATCCTGGGTTTTGTCGTAGGTGCCGTGTCCCCAGCTGTCGTGGTGCCCTCCATGCTCCTTTTGCA
  GGAAGGAGGCTACGGTGTTGGAAAAGGTATCCCAACCTTACTCATGGCCGCCGGCAGCTTCGATGACATC
  CTGGCCATCACTGGCTTCAACACGTGCTTAGGCGTGGCCTTTTCCACAGGATCTACAGTTTTTAACATCT
  TCAGAGGCATCTTGGAGGTGGTAATTGGTGTGGCAGCTGGATCTTTTCTTGGGTTTTTTATCCAGTACTT
  CCCGAGCAGGGACCAGGACAACCTCGTGTGGAAGCGAGCCTTTCTGGTTCTGGGTTTTGCTGTGCTCGCT
  GTGTTCAGCAGTGTGTATTTTAGCTTCCCGGGGTCTGGAGGACTCTGCACGTTGGTCATGGCTTTCCTAG
  CAGGCATGAGGTGGACTGACAAGAAGTCAGAGGTAGAAAAGGTCATTGCAGTTACCTGGGACGTTTTCCA
  GCCTCTTCTTTTTGGCCTGATTGGAGCAGAGGTTTCCATTGTGTCTCTCAGAGCAGAAACGGTTGGCCTT
  TGTGTTGCAACCCTCAGCATCGCAGTGCTTATACGAATTCTGACTACATTCCTGATGGTGTGTTTCGCTG
  GCTTTAACATAAAGGAAAAGATATTTATTTCTTTTGCCTGGCTTCCAAAGGCCACGGTCCAGGCTGCCAT
  TGGCTCTGTGGCTCTGGACACGGCAAGATCCCACGGAGAGAAGCAGCTGGAAGACTATGGGATGGATGTG
  CTGACGGTGGCATTTTTGGCCATCCTCATTACAGCACCAATTGGAAGCCTACTGATTGGTTTGCTGGGTC
  CCAGGGTTCTTCAGAAATCTGAACATCGAACCGAAGAGGAGGTTCAAGGAGAGACTTCTGCACACATTCA
  GAGGAAGCCTGAGGATTCCATTACGGAAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178877
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178877.6, NP_849208.4
• RefSeq Size: 2243 bp
• RefSeq ORF: 1644 bp
• Locus ID: 97086
• UniProt ID: Q5BKR2
• Cytogenetics: 3 G3
• Gene Summary: Na(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane (PubMed:18508966, PubMed:17988971). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (PubMed:23720317). Involved in sperm motility and fertility (PubMed:27010853). It is controversial whether SLC9B2 plays a role in osteoclast differentiation (PubMed:17988971) or not (PubMed:20441802, PubMed:22985540).[UniProtKB/Swiss-Prot Function]