Zkscan17 (NM_001291014) Mouse Untagged Clone

CAT#: MC227658

Zkscan17 (untagged) - Mouse zinc finger with KRAB and SCAN domains 17 (Zkscan17), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zkscan17
• Synonyms: Nizp1; Zfp496; Znf496
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227658 representing NM_001291014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCAGAGTCCCAGAGCTACCCTGATGCCCCGCCTGGGGCTCTAGTACAGGGCACTGGGCTTCTAA
  GCAGATCACCAGGCCAGCCTAGCGAAGACTTAGTTCCGCAAGATGCGTTCGTTGTGCAGGAGCAGAGCAT
  CAGGGATGCCCAGCCCGTGGCCACCTGCCAGCTTCCCCCGAACCGAGTCTCCCCATTTAAGGACATGATT
  TTATGCTTCTCAGAAGAGGACTGGTCCCTTCTGGACCCGGCACAGACTGGTTTCTATGGCGAATTCATCA
  TTGGGGAAGACTATGCAGTCTCCATGCCTCCAAATGAGCCTCCAGTTCAGCCAGGCCACTCCCATGAAGA
  GGAGAACGGGTTGCGGGTCACAGAGTGGACGACAGACCTCCAGGATAAAGAAATCCCCCAGGCCTCCTGC
  TTAGACCTTTCCAGCCTCCAGCCATTCCAAGGAGAAGAAAGAAGGAAGTGGGAGGAGCTGCAGGTGCCAG
  AGCTCCAGCCCTGCCCACAGGTGGTGCTCAGTCAGAGTCCCTGTCCAGCAGGAGGTGACCCGCCAGCCCT
  CAAGAGCAGTCTGGACCAGGAGGTGACCATCGAGATCGTGCTGTCCAGCTCTGGGGATGAGGACTCCCAG
  CACAGCCCATACTGCACAGAGGAGCTGAGGAGCCCTCCAGAAGACCTGCACAGTGTCCCAGCCCACCAGA
  GCAACGCTAGTGCTGAGGGGGAGGTGCAAACCTCACAGAAATCTTACGTGTGCCCAAACTGTGGAAAGAT
  CTTCCGCTGGAGGGTCAACTTCATCCGGCACCTGCGCAGCCGCAGGGAGCAGAAGCCACACAAATGCTCA
  GTGTGCGGGGAGTTATTCAGTGACAGCGAGGACCTGGATGGTCACCTAGAGACCCACGAGGCCCAGAAGC
  CCTACAGGTGCACTGCCTGCGGGAAGAGCTTCCGCCTCAACTCACACCTCATCTCTCACCGCCGGATCCA
  CCTGCAGCCAGCCAGCCAGCAGCCCATGAAGAAGAGTGAGGAAGAAGCTCTGGAAACAGAGGGCACGGGT
  GCCAGCGACCTGCTGGAGAAGAGCAAAGCCAAGCTGAGCTTCCAGTGTGGGGACTGTGAGAAGAGCTTCC
  AGCGGCATGACCATCTGGTGAGACACCGTAGACACTGTCATCTAAAGGATGAGACCCGTCCCTTCCAGTG
  TCGGTACTGTGTCAAAACTTTCCGACAGAATTATGACCTCCTCCGCCATGAGCGCCTGCACATGAAGCGA
  CGCTCCAAGCAGGCTCTCAACTCATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291014
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291014.1, NP_001277943.1
• RefSeq Size: 3781 bp
• RefSeq ORF: 1290 bp
• Locus ID: 268417
• UniProt ID: Q5SXI5
• Cytogenetics: 11 B1.3
• Gene Summary: DNA-binding transcription factor that can both act as an activator and a repressor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in its 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.