Gapvd1 (NM_025709) Mouse Untagged Clone

CAT#: MC224462

Gapvd1 (untagged) - Mouse GTPase activating protein and VPS9 domains 1 (Gapvd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gapvd1
• Synonyms: 2010005B09Rik; 4432404J10Rik; AW108497; Gapex-5; mKIAA1521; RAP6; RME-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224462 representing NM_025709
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCTAGATATTCACACATTGGCCCACCACCTCAAGCAGGAACGCTTATATGTGAGCTCTGAGA
  AACAGCTCATTCAGAGGCTAAATGCCGATGTACTGAAGACAGCTGAAAAGCTGTATCGAACAGCGTGGAT
  TGCAAAGCAACAGAGGATTAATTTGGATCGTCTTATTATAACCAGTGCTGAAGCTTCCCCTGCTGAGTGT
  TGCCAGCATGCCAAGATTTTGGAAGATACACAGTTTGTTGATGGATATAAGCAACTGGGATTTCAGGAAA
  CAGCTTATGGAGAATTCCTGAGTCGGTTAAGAGAAAATCCACGACTTATTGCCTCCTCTTTGGTTGCTGG
  AGAAAAACTCAATCAGGAGAACACACAAAGTGTTATCTACACTGTTTTTACCTCCTTGTATGGCAACTGT
  ATTATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTACGATACTTGATTGAATTTGAACTTAAAGAAA
  GTGACAACCCCAGGCGCCTTTTGAGGAGAGGAACTTGTGCCTTCAGCATCTTATTTAAACTTTTTTCTGA
  AGGACTGTTTTCTGCCAAACTTTTCCTTACGGCTACTTTGCATGAGCCAATCATGCAGCTCCTCGTAGAG
  GATGAAGACCACCTGGAAACAGATCCAAACAAGCTGATAGAGAGATTCTCTCCAGCTCAGCAAGAAAAAC
  TCTTTGGAGAGAAAGGCTCAGATAGATTTAGGCAAAAGGTTCAAGAAATGGTAGATTCCAATGAGGCTAA
  ACTAGTGGCTTTGGTGAACAAATTTATTGGTTATCTTAAACAGAATACATACTGCTTTCCACATAGTTTG
  AGGTGGATTGTGTCACAGATGTACAAAACTCTTTCTTGTGTAGACAGATTGGAGGTTGGGGAAGTCAGAG
  CAATGTGTACCGATCTTCTGCTGGCTTGCTTCATTTGTCCTGCAGTTGTCAATCCAGAGCAGTATGGGAT
  AATATCTGATGCTCCTATTAATGAGGTGGCAAGATTTAACCTGATGCAGGTTGGCCGCCTTTTGCAGCAG
  CTAGCAATGACTGGCACTGAAGAAGGAGATCCCCGGACCAAAAATAGCCTTGGAAAATTTGACAAAGGCT
  GTGTCGCTGCTTTCCTTGATGTTGTAATTGGGGGCCGTGCAGTGGAGACTCCGCCAATGTCCTCTGTTAA
  TCTTCTGGAAGGCTTGAGCAGAACTGTGGTGTATATAAGTTACAGTCAGCTTATTACTCTGGTAAATTTT
  ATGAAGAGTGTGATGTCTGGAGATCAACTGAAAGAAGATAGAATGGCTCTTGACAATTTGTTGGCAAACC
  TGCCCCAGGCTAAGCCAGGGAAGAGCAGCAGTCTGGACATGACTCCCTATAGCACTCCTCAGATGTCTCC
  AGCAACCACTCCAGCCAACAAGAAGAATCGATTACCTATAGCAACTCGGAGCAGAAGCCGAAGTAATATG
  TTAATGGACCTACATATGGATCATGAAGGATCATCTCAAGAAACCATCCAGGAGGTACAGCCAGAAGAGG
  TGCTGGTCATTTCCTTAGGGACAGGCCCTCAGCTCACTCCAGGGATGATGTCAGAAAATGAGGTTCTAAA
  CATGCAGCTTTCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAAAACAAACTCCACGGTCCTTCCAAT
  CGCTCCAATTCGGTGTCTTCTCTCGACCTGGAGGGAGAGTCTGTATCTGAACTTGGGGCAGGACCTTCTG
  GGAGTAACGGAGTTGAAGCACTGCAGCTGTTAGAGCACGAGCAAGCTACAACACAAGATAATCTTGATGA
  TAAACTGAGGAAGTTTGAGATTCGTGACATGATGGGACTAACAGATGACAGGGACATATCCGAGACTGTG
  AGTGAGACCTGGAGCACTGATGTCCTGGGAAGTGACTTCGACCCGAACGTTGATGAAGATCGTTTGCAAG
  AGATTGCAGGTGCAGCAGCAGAGAACGTGTTAGGTAGTTTGCTGTGCCTTCCAGGTTCAGGGTCAGTTCT
  CCTTGACCCTTGCACTGGCTCTACCATATCAGAGACCACAAGTGAAGCTTGGAGTGTAGAGGTATTGCCA
  AGTGACTCAGAGGCTCCAGACTTAAAGCAGGAAGAGCGCCTCCAAGAGCTGGAGAGCTGTTCTGGACTGG
  GCAGCACATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGCCCCAGCACACCAGGCCTCAGTGTTGT
  GTCCGGTATAAGTGCTACATCGGAGGATATTCCCAATAAGATCGAAGACCTGAGATCTGAGTGCAGCTCT
  GATTTTGGGGGCAAAGATTCTGTAACTAGTCCAGATATGGATGACATAGCCCATGGCGCTCATCAGCTGA
  CCTCTCCTCCCTCTCAGTCAGAGTCTCTGCTTGCCATGTTTGATCCACTGTCCTCACATGAAGGGGCCTC
  TGCTGTGGTAAGGCCAAAGGTTCACTATGCCCGACCCTCGCATCCACCTCCGGATCCTCCAATCCTGGAA
  GGTGCAGTGGGAGGAAATGAGGCCAGGTTGCCAAACTTTGGCTCTCACGTTTTAACTGCAGCTGAAATGG
  AGGCGTTCAAGCAGAGACACTCATACCCTGAGAGATTGGTTCGCAGCAGAAGCTCTGATATAGTGTCTTC
  TGTGCGGCGGCCCATGAGTGACCCCAGCTGGAACCGACGTCCAGGGAATGAAGAACTTCCTCCAGCTGCA
  GCCACTGGTGCTACTTCTTTGGTGGCTGCACCTCACTCGTCATCTTCGTCCCCGAGTAAGGACTCTTCTA
  GAGGAGAGACTGAGGAACGCAAGGACAGTGACGATGAGAGGTCAGACAGGAGCAGGCCTTGGTGGAGGAA
  GCGCTTTGTTTCAGCTATGCCTAAAGCTCCTATACCATTTAGAAAGAAAGAAAAACAAGAAAAAGACAAA
  GATGATCTGGGTCCTGACAGATTCTCAACACTCACAGATGAGCCCAGCCCTAGGCTCAGTGCTCAAGCTC
  AGGTTGCTGAAGATATTCTGGACAAGTACCGGAATGCCATTAAACGGACCAGCCCCAGTGAAGGAGCAAT
  GGCAAATGATGAGAGTGCAGAGGTCATGGGTGATGGTGAAAGTGCCCACGACTCTCCTCGTGAGGAAGCA
  CTGCAGAACATCTCAGCCGACGACCTCCCGGACTCCGCCAGCCAGGCAGCCCACCCTCAGGACTCTGCTT
  TCTCTTACAGAGATGTAAAAAAGAAGCTGAGGCTCGCACTTTGCTCTGCAGACTCTGTGGCCTTCCCAGT
  GCTAACTCATTCAACAAGGAATGGATTGCCAGACCACACAGACCCAGAAGACAATGAAATTGTATGCTTC
  TTAAAAGTTCAAATAGCTGAAGCAATTAATTTACAAGATAAGAGCTTAATGGCTCAACTTCAAGAAACAA
  TGCGTTGTGTGTGCCGCTTTGACAATAGGACTTGTAGGAAGTTGCTGGCATCCATTGCTGAAGACTACAG
  AAAAAGAGCTCCGTATATTGCTTATCTTACCCGCTGCCGACAAGGACTGCAGACCACACAGGCTCACTTG
  GAAAGGCTCTTGCAAAGAGTTTTGCGGGACAAAGAGGTGGCCAATCGGTACTTCACCACTGTCTGTGTGC
  GGTTACTGCTTGAGAGCAAAGAAAAGAAAATCAGGGAGTTCATTCAAGACTTTCAGAAACTTACTGCTGC
  TGATGATAAAACGGCTCAGGTGGAGGATTTTCTACAGTTCCTGTATGGTGTGATGGCTCAGGATGTCATA
  TGGCAGAATGCCAGTGAGGAGCAGCTGCAGGATGCCCAGCTGGCCATCGAGCGGAGTGTGATGAATCGGA
  TCTTCAAGCTTGCTTTCTACCCTAACCAGGATGGGGACATACTTCGTGACCAGGTTCTGCATGAACATAT
  CCAGAGATTATCTAAAGTAGTGACTGCAAACCACAGAGCTCTGCAGATCCCTGAGGTTTATCTCCGTGAG
  GCACCATGGCCATCTGCACAGTCAGAAATCAGGACAATAAGTGCTTACAAAACGCCCCGCGACAAAGTGC
  AGTGCATCCTGAGGATGTGCTCCACCATCATGAACCTCTTGAGCCTGGCCAACGAGGACTCTGTCCCCGG
  GGCAGATGACTTTGTTCCTGTGTTGGTGTTTGTGTTAATAAAGGCAAACCCACCCTGCCTGCTGTCCACT
  GTGCAGTACATCAGCAGCTTCTATGCCAGCTGTCTGTCTGGAGAGGAGTCCTACTGGTGGATGCAGTTCA
  CAGCAGCCGTGGAGTTTATTAAAACCATCGATGACAGAAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025709
• Insert Size: 4314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025709.2, NP_079985.2
• RefSeq Size: 5966 bp
• RefSeq ORF: 4314 bp
• Locus ID: 66691
• UniProt ID: Q6PAR5
• Cytogenetics: 2 B
• Gene Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.