Gapvd1 (NM_025709) Mouse Tagged ORF Clone

CAT#: MG211988

Gapvd1 (tGFP-tagged) - Mouse GTPase activating protein and VPS9 domains 1 (Gapvd1)

Product Images

Circular map for MG211988

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gapvd1
• Synonyms: 2010005B09Rik; 4432404J10Rik; AW108497; Gapex-5; mKIAA1521; RAP6; RME-6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211988 representing NM_025709
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCTAGATATTCACACATTGGCCCACCACCTCAAGCAGGAACGCTTATATGTGAGCTCTGAGA
  AACAGCTCATTCAGAGGCTAAATGCCGATGTACTGAAGACAGCTGAAAAGCTGTATCGAACAGCGTGGAT
  TGCAAAGCAACAGAGGATTAATTTGGATCGTCTTATTATAACCAGTGCTGAAGCTTCCCCTGCTGAGTGT
  TGCCAGCATGCCAAGATTTTGGAAGATACACAGTTTGTTGATGGATATAAGCAACTGGGATTTCAGGAAA
  CAGCTTATGGAGAATTCCTGAGTCGGTTAAGAGAAAATCCACGACTTATTGCCTCCTCTTTGGTTGCTGG
  AGAAAAACTCAATCAGGAGAACACACAAAGTGTTATCTACACTGTTTTTACCTCCTTGTATGGCAACTGT
  ATTATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTACGATACTTGATTGAATTTGAACTTAAAGAAA
  GTGACAACCCCAGGCGCCTTTTGAGGAGAGGAACTTGTGCCTTCAGCATCTTATTTAAACTTTTTTCTGA
  AGGACTGTTTTCTGCCAAACTTTTCCTTACGGCTACTTTGCATGAGCCAATCATGCAGCTCCTCGTAGAG
  GATGAAGACCACCTGGAAACAGATCCAAACAAGCTGATAGAGAGATTCTCTCCAGCTCAGCAAGAAAAAC
  TCTTTGGAGAGAAAGGCTCAGATAGATTTAGGCAAAAGGTTCAAGAAATGGTAGATTCCAATGAGGCTAA
  ACTAGTGGCTTTGGTGAACAAATTTATTGGTTATCTTAAACAGAATACATACTGCTTTCCACATAGTTTG
  AGGTGGATTGTGTCACAGATGTACAAAACTCTTTCTTGTGTAGACAGATTGGAGGTTGGGGAAGTCAGAG
  CAATGTGTACCGATCTTCTGCTGGCTTGCTTCATTTGTCCTGCAGTTGTCAATCCAGAGCAGTATGGGAT
  AATATCTGATGCTCCTATTAATGAGGTGGCAAGATTTAACCTGATGCAGGTTGGCCGCCTTTTGCAGCAG
  CTAGCAATGACTGGCACTGAAGAAGGAGATCCCCGGACCAAAAATAGCCTTGGAAAATTTGACAAAGGCT
  GTGTCGCTGCTTTCCTTGATGTTGTAATTGGGGGCCGTGCAGTGGAGACTCCGCCAATGTCCTCTGTTAA
  TCTTCTGGAAGGCTTGAGCAGAACTGTGGTGTATATAAGTTACAGTCAGCTTATTACTCTGGTAAATTTT
  ATGAAGAGTGTGATGTCTGGAGATCAACTGAAAGAAGATAGAATGGCTCTTGACAATTTGTTGGCAAACC
  TGCCCCAGGCTAAGCCAGGGAAGAGCAGCAGTCTGGACATGACTCCCTATAGCACTCCTCAGATGTCTCC
  AGCAACCACTCCAGCCAACAAGAAGAATCGATTACCTATAGCAACTCGGAGCAGAAGCCGAAGTAATATG
  TTAATGGACCTACATATGGATCATGAAGGATCATCTCAAGAAACCATCCAGGAGGTACAGCCAGAAGAGG
  TGCTGGTCATTTCCTTAGGGACAGGCCCTCAGCTCACTCCAGGGATGATGTCAGAAAATGAGGTTCTAAA
  CATGCAGCTTTCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAAAACAAACTCCACGGTCCTTCCAAT
  CGCTCCAATTCGGTGTCTTCTCTCGACCTGGAGGGAGAGTCTGTATCTGAACTTGGGGCAGGACCTTCTG
  GGAGTAACGGAGTTGAAGCACTGCAGCTGTTAGAGCACGAGCAAGCTACAACACAAGATAATCTTGATGA
  TAAACTGAGGAAGTTTGAGATTCGTGACATGATGGGACTAACAGATGACAGGGACATATCCGAGACTGTG
  AGTGAGACCTGGAGCACTGATGTCCTGGGAAGTGACTTCGACCCGAACGTTGATGAAGATCGTTTGCAAG
  AGATTGCAGGTGCAGCAGCAGAGAACGTGTTAGGTAGTTTGCTGTGCCTTCCAGGTTCAGGGTCAGTTCT
  CCTTGACCCTTGCACTGGCTCTACCATATCAGAGACCACAAGTGAAGCTTGGAGTGTAGAGGTATTGCCA
  AGTGACTCAGAGGCTCCAGACTTAAAGCAGGAAGAGCGCCTCCAAGAGCTGGAGAGCTGTTCTGGACTGG
  GCAGCACATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGCCCCAGCACACCAGGCCTCAGTGTTGT
  GTCCGGTATAAGTGCTACATCGGAGGATATTCCCAATAAGATCGAAGACCTGAGATCTGAGTGCAGCTCT
  GATTTTGGGGGCAAAGATTCTGTAACTAGTCCAGATATGGATGACATAGCCCATGGCGCTCATCAGCTGA
  CCTCTCCTCCCTCTCAGTCAGAGTCTCTGCTTGCCATGTTTGATCCACTGTCCTCACATGAAGGGGCCTC
  TGCTGTGGTAAGGCCAAAGGTTCACTATGCCCGACCCTCGCATCCACCTCCGGATCCTCCAATCCTGGAA
  GGTGCAGTGGGAGGAAATGAGGCCAGGTTGCCAAACTTTGGCTCTCACGTTTTAACTGCAGCTGAAATGG
  AGGCGTTCAAGCAGAGACACTCATACCCTGAGAGATTGGTTCGCAGCAGAAGCTCTGATATAGTGTCTTC
  TGTGCGGCGGCCCATGAGTGACCCCAGCTGGAACCGACGTCCAGGGAATGAAGAACTTCCTCCAGCTGCA
  GCCACTGGTGCTACTTCTTTGGTGGCTGCACCTCACTCGTCATCTTCGTCCCCGAGTAAGGACTCTTCTA
  GAGGAGAGACTGAGGAACGCAAGGACAGTGACGATGAGAGGTCAGACAGGAGCAGGCCTTGGTGGAGGAA
  GCGCTTTGTTTCAGCTATGCCTAAAGCTCCTATACCATTTAGAAAGAAAGAAAAACAAGAAAAAGACAAA
  GATGATCTGGGTCCTGACAGATTCTCAACACTCACAGATGAGCCCAGCCCTAGGCTCAGTGCTCAAGCTC
  AGGTTGCTGAAGATATTCTGGACAAGTACCGGAATGCCATTAAACGGACCAGCCCCAGTGAAGGAGCAAT
  GGCAAATGATGAGAGTGCAGAGGTCATGGGTGATGGTGAAAGTGCCCACGACTCTCCTCGTGAGGAAGCA
  CTGCAGAACATCTCAGCCGACGACCTCCCGGACTCCGCCAGCCAGGCAGCCCACCCTCAGGACTCTGCTT
  TCTCTTACAGAGATGTAAAAAAGAAGCTGAGGCTCGCACTTTGCTCTGCAGACTCTGTGGCCTTCCCAGT
  GCTAACTCATTCAACAAGGAATGGATTGCCAGACCACACAGACCCAGAAGACAATGAAATTGTATGCTTC
  TTAAAAGTTCAAATAGCTGAAGCAATTAATTTACAAGATAAGAGCTTAATGGCTCAACTTCAAGAAACAA
  TGCGTTGTGTGTGCCGCTTTGACAATAGGACTTGTAGGAAGTTGCTGGCATCCATTGCTGAAGACTACAG
  AAAAAGAGCTCCGTATATTGCTTATCTTACCCGCTGCCGACAAGGACTGCAGACCACACAGGCTCACTTG
  GAAAGGCTCTTGCAAAGAGTTTTGCGGGACAAAGAGGTGGCCAATCGGTACTTCACCACTGTCTGTGTGC
  GGTTACTGCTTGAGAGCAAAGAAAAGAAAATCAGGGAGTTCATTCAAGACTTTCAGAAACTTACTGCTGC
  TGATGATAAAACGGCTCAGGTGGAGGATTTTCTACAGTTCCTGTATGGTGTGATGGCTCAGGATGTCATA
  TGGCAGAATGCCAGTGAGGAGCAGCTGCAGGATGCCCAGCTGGCCATCGAGCGGAGTGTGATGAATCGGA
  TCTTCAAGCTTGCTTTCTACCCTAACCAGGATGGGGACATACTTCGTGACCAGGTTCTGCATGAACATAT
  CCAGAGATTATCTAAAGTAGTGACTGCAAACCACAGAGCTCTGCAGATCCCTGAGGTTTATCTCCGTGAG
  GCACCATGGCCATCTGCACAGTCAGAAATCAGGACAATAAGTGCTTACAAAACGCCCCGCGACAAAGTGC
  AGTGCATCCTGAGGATGTGCTCCACCATCATGAACCTCTTGAGCCTGGCCAACGAGGACTCTGTCCCCGG
  GGCAGATGACTTTGTTCCTGTGTTGGTGTTTGTGTTAATAAAGGCAAACCCACCCTGCCTGCTGTCCACT
  GTGCAGTACATCAGCAGCTTCTATGCCAGCTGTCTGTCTGGAGAGGAGTCCTACTGGTGGATGCAGTTCA
  CAGCAGCCGTGGAGTTTATTAAAACCATCGATGACAGAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211988 representing NM_025709
  Red=Cloning site Green=Tags(s)
  
  MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAEC
  CQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNC
  IMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVE
  DEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSL
  RWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQ
  LAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNF
  MKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNM
  LMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSN
  RSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETV
  SETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLP
  SDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSS
  DFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILE
  GAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAA
  ATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDK
  DDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEA
  LQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCF
  LKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHL
  ERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVI
  WQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLRE
  APWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLST
  VQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025709
• ORF Size: 4311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025709.3
• RefSeq Size: 5966 bp
• RefSeq ORF: 4314 bp
• Locus ID: 66691
• UniProt ID: Q6PAR5
• Cytogenetics: 2 B
• Gene Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]