Scube1 (NM_022723) Mouse Untagged Clone

CAT#: MC222998

Scube1 (untagged) - Mouse signal peptide, CUB domain, EGF-like 1 (Scube1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scube1
• Synonyms: 7330410C13Rik; A630023E24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222998 representing NM_022723
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGGCGGCCGTGCGCTGGCACTTGTACCTACTGCTCGCTCTGGGAGCGCGCGGGCGGCTGGTGG
  GGGGCAGCGGGCTGCCAGGAGCCGTGGACGTGGATGAATGCTCAGAGGGCACAGACGACTGCCACATAGA
  TGCCATCTGCCAGAACACACCCAAGTCTTACAAATGTCTCTGCAAGCCCGGCTATAAGGGGGAAGGAAGG
  CAGTGTGAAGACATTGACGAGTGTGAGAATGACTACTACAACGGGGGCTGTGTCCATGACTGCATCAACA
  TCCCTGGGAACTACAGGTGCACCTGCTTTGATGGCTTCATGCTGGCCCATGATGGACACAACTGCCTGGA
  TGTAGATGAGTGTCAAGACAACAACGGCGGCTGCCAGCAGATCTGCGTCAATGCCATGGGCAGCTATGAA
  TGCCAGTGCCACAGCGGCTTTTTTCTCAGTGACAACCAACACACCTGCATACACCGCTCCAATGAGGGTA
  TGAACTGCATGAACAAAGACCATGGCTGTGCCCACATCTGCCGGGAGACGCCCAAAGGTGGGGTAGCCTG
  TGACTGCAGGCCCGGCTTTGACCTTGCCCAAAACCAGAAGGACTGCACACTAACCTGTAACTATGGAAAC
  GGAGGCTGTCAGCACAGCTGCGAGGACACGGACACGGGCCCCATGTGTGGCTGCCACCAGAAGTACGCCT
  TACATGCAGATGGTCGGACGTGCATCGAGACATGTGCAGTGAACAACGGAGGCTGTGACCGGACGTGTAA
  GGACACTGCCACTGGCGTGCGGTGCAGCTGCCCGGTTGGATTCACGCTGCAGCCAGATGGAAAGACATGC
  AAAGACATCAACGAGTGCTTGATGAATAACGGAGGCTGCGACCACTTCTGCCGAAACACCGTGGGCAGCT
  TTGAATGCGGCTGCCAAAAGGGCCACAAGCTACTGACGGACGAGCGGACATGCCAAGACATTGACGAGTG
  TTCCTTTGAGCGGACCTGTGACCACATCTGCATCAACTCCCCCGGCAGTTTCCAGTGCCTGTGCCGCCGA
  GGCTACACACTCTACGGGACAACCCACTGCGGAGACGTGGATGAATGTAGCATGAACAACGGCAGCTGTG
  AGCAGGGCTGTGTCAACACCAAGGGCAGCTACGAGTGTGTGTGCCCACCAGGGAGGAGGCTGCACTGGAA
  TCAGAAGGACTGTGTGGAGATGAACGGGTGCCTGTCTCGGTCCAAGGCCTCTGCCCAGGCCCAGCTGTCC
  TGTGGCAAGGTGGGTGGAGTGGAAAACTGCTTCCTGTCCTGCCTGGGCCACAGTCTCTTCATGCCAGATT
  CAGAAAGCAGCTACATCCTGAGCTGTGGTGTTCCAGGTCTCCAGGGCAAGACACTGCCAAAACGCAATGG
  CACTAGCTCCAGTACAGGGCCCGGCTGCTCAGATGCCCCCACCACCCCTATCAGACAGAAGGCCCGCTTC
  AAGATCAGAGATGCCAAGTGCCATCTCCAGCCCCGCAGCCAGGAGCGAGCCAAGGATACCCTGAGGCATC
  CCCTTTTAGACAACTGCCACGTGACTTTTGTGACCCTCAAGTGTGACTCCTCCAAGAAGAGGCGCCGAGG
  CCGCAAGTCCCCATCCAAGGAGGTGTCCCACATCACAGCAGAGTTTGAGGTGGAGATGAAGGTGGATGAG
  GCCTCAGGCACCTGCGAAGCAGACTGCATGCGGAAGCGTGCCGAGCAGAGCCTGCAAGCCGCCATCAAGA
  TTCTGCGCAAGTCCATTGGTCGGAACCAGTTCTATGTCCAGGTCTTGGGCACTGAGTACGAGGTGGCCCA
  GCGGCCAGCCAAAGCACTGGAGGGAACCGGGACTTGCGGCATAGGCCAAATACTACAGGACGGCAAGTGT
  GTACCGTGTGCTCCTGGCACCTACTTCAGTGGTGACCCAGGCCAGTGTATGCCATGCGTGTCGGGAACCT
  ACCAGGACATGGAAGGCCAGCTCAGCTGCACGCCATGCCCCAGCAGCGAAGGACTGGGTCTGGCTGGTGC
  CCGAAACGTATCCGAATGTGGAGGCCAGTGCTCTCCAGGCTACTTCTCAGCGGATGGGTTCAAGCCTTGC
  CAGGCCTGCCCTGTGGGCACATACCAGCCCGAGCCTGGGCGAACCGGCTGCTTCCCCTGTGGAGGCGGGC
  TGCTCACCAAACACACGGGTACCGCCTCTTTCCAGGACTGCGAGGCCAAAGTGCATTGCTCTCCCGGACA
  CCACTACAATACCACCACCCACCGGTGTATCCGCTGTCCCGTGGGCACCTATCAGCCCGAGTTTGGCCAG
  AACCATTGCATCTCCTGTCCGGGCAACACCAGCACCGATTTTGATGGTTCCACCAACGTCACACACTGCA
  AGAACCAGCACTGCGGCGGCGAACTTGGTGACTACACAGGCTACATCGAGTCCCCCAACTACCCTGGCGA
  CTACCCAGCCAATGCAGAGTGTGTGTGGCACATCGCACCTCCCCCAAAGCGCAGGATCCTAATCGTAGTC
  CCGGAGATCTTCCTACCCATCGAGGACGAGTGTGGTGATGTCCTGGTCATGAGAAAGAGTGCCTCGCCCA
  CGTCAGTCACCACCTATGAGACGTGCCAGACCTATGAGCGGCCCATCGCCTTTACCTCCCGTTCCCGCAA
  ACTCTGGATCCAGTTCAAATCCAACGAAGCCAACAGTGGCAAAGGGTTTCAAGTGCCCTATGTCACCTAT
  GATGAGGACTACCAGCAGCTAATAGAAGACATCGTGCGTGATGGGCGCTTATATGCCTCGGAGAACCATC
  AGGAGATTTTGAAAGACAAGAAGCTGATCAAGGCCCTCTTTGACGTGCTGGCACACCCACAAAACTACTT
  CAAATACACAGCCCAGGAGTCCAAGGAGATGTTCCCCCGGTCCTTTATCAAACTTCTGCGTTCCAAAGTG
  TCTCGGTTCCTGCGGCCCTACAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022723
• Insert Size: 2967 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022723.3, NP_073560.2
• RefSeq Size: 7842 bp
• RefSeq ORF: 2967 bp
• Locus ID: 64706
• UniProt ID: Q6NZL8
• Cytogenetics: 15 E1
• Gene Summary: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.