Scube1 (NM_022723) Mouse Tagged ORF Clone

CAT#: MG225502

Scube1 (tGFP-tagged) - Mouse signal peptide CUB domain EGF-like 1 (Scube1), (10ug)

Product Images

Circular map for MG225502

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Scube1
• Synonyms: 7330410C13Rik; A630023E24Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225502 representing NM_022723
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGGCGGCCGTGCGCTGGCACTTGTACCTACTGCTCGCTCTGGGAGCGCGCGGGCGGCTGGTGG
  GGGGCAGCGGGCTGCCAGGAGCCGTGGACGTGGATGAATGCTCAGAGGGCACAGACGACTGCCACATAGA
  TGCCATCTGCCAGAACACACCCAAGTCTTACAAATGTCTCTGCAAGCCCGGCTATAAGGGGGAAGGAAGG
  CAGTGTGAAGACATTGACGAGTGTGAGAATGACTACTACAACGGGGGCTGTGTCCATGACTGCATCAACA
  TCCCTGGGAACTACAGGTGCACCTGCTTTGATGGCTTCATGCTGGCCCATGATGGACACAACTGCCTGGA
  TGTAGATGAGTGTCAAGACAACAACGGCGGCTGCCAGCAGATCTGCGTCAATGCCATGGGCAGCTATGAA
  TGCCAGTGCCACAGCGGCTTTTTTCTCAGTGACAACCAACACACCTGCATACACCGCTCCAATGAGGGTA
  TGAACTGCATGAACAAAGACCATGGCTGTGCCCACATCTGCCGGGAGACGCCCAAAGGTGGGGTAGCCTG
  TGACTGCAGGCCCGGCTTTGACCTTGCCCAAAACCAGAAGGACTGCACACTAACCTGTAACTATGGAAAC
  GGAGGCTGTCAGCACAGCTGCGAGGACACGGACACGGGCCCCATGTGTGGCTGCCACCAGAAGTACGCCT
  TACATGCAGATGGTCGGACGTGCATCGAGACATGTGCAGTGAACAACGGAGGCTGTGACCGGACGTGTAA
  GGACACTGCCACTGGCGTGCGGTGCAGCTGCCCGGTTGGATTCACGCTGCAGCCAGATGGAAAGACATGC
  AAAGACATCAACGAGTGCTTGATGAATAACGGAGGCTGCGACCACTTCTGCCGAAACACCGTGGGCAGCT
  TTGAATGCGGCTGCCAAAAGGGCCACAAGCTACTGACGGACGAGCGGACATGCCAAGACATTGACGAGTG
  TTCCTTTGAGCGGACCTGTGACCACATCTGCATCAACTCCCCCGGCAGTTTCCAGTGCCTGTGCCGCCGA
  GGCTACACACTCTACGGGACAACCCACTGCGGAGACGTGGATGAATGTAGCATGAACAACGGCAGCTGTG
  AGCAGGGCTGTGTCAACACCAAGGGCAGCTACGAGTGTGTGTGCCCACCAGGGAGGAGGCTGCACTGGAA
  TCAGAAGGACTGTGTGGAGATGAACGGGTGCCTGTCTCGGTCCAAGGCCTCTGCCCAGGCCCAGCTGTCC
  TGTGGCAAGGTGGGTGGAGTGGAAAACTGCTTCCTGTCCTGCCTGGGCCACAGTCTCTTCATGCCAGATT
  CAGAAAGCAGCTACATCCTGAGCTGTGGTGTTCCAGGTCTCCAGGGCAAGACACTGCCAAAACGCAATGG
  CACTAGCTCCAGTACAGGGCCCGGCTGCTCAGATGCCCCCACCACCCCTATCAGACAGAAGGCCCGCTTC
  AAGATCAGAGATGCCAAGTGCCATCTCCAGCCCCGCAGCCAGGAGCGAGCCAAGGATACCCTGAGGCATC
  CCCTTTTAGACAACTGCCACGTGACTTTTGTGACCCTCAAGTGTGACTCCTCCAAGAAGAGGCGCCGAGG
  CCGCAAGTCCCCATCCAAGGAGGTGTCCCACATCACAGCAGAGTTTGAGGTGGAGATGAAGGTGGATGAG
  GCCTCAGGCACCTGCGAAGCAGACTGCATGCGGAAGCGTGCCGAGCAGAGCCTGCAAGCCGCCATCAAGA
  TTCTGCGCAAGTCCATTGGTCGGAACCAGTTCTATGTCCAGGTCTTGGGCACTGAGTACGAGGTGGCCCA
  GCGGCCAGCCAAAGCACTGGAGGGAACCGGGACTTGCGGCATAGGCCAAATACTACAGGACGGCAAGTGT
  GTACCGTGTGCTCCTGGCACCTACTTCAGTGGTGACCCAGGCCAGTGTATGCCATGCGTGTCGGGAACCT
  ACCAGGACATGGAAGGCCAGCTCAGCTGCACGCCATGCCCCAGCAGCGAAGGACTGGGTCTGGCTGGTGC
  CCGAAACGTATCCGAATGTGGAGGCCAGTGCTCTCCAGGCTACTTCTCAGCGGATGGGTTCAAGCCTTGC
  CAGGCCTGCCCTGTGGGCACATACCAGCCCGAGCCTGGGCGAACCGGCTGCTTCCCCTGTGGAGGCGGGC
  TGCTCACCAAACACACGGGTACCGCCTCTTTCCAGGACTGCGAGGCCAAAGTGCATTGCTCTCCCGGACA
  CCACTACAATACCACCACCCACCGGTGTATCCGCTGTCCCGTGGGCACCTATCAGCCCGAGTTTGGCCAG
  AACCATTGCATCTCCTGTCCGGGCAACACCAGCACCGATTTTGATGGTTCCACCAACGTCACACACTGCA
  AGAACCAGCACTGCGGCGGCGAACTTGGTGACTACACAGGCTACATCGAGTCCCCCAACTACCCTGGCGA
  CTACCCAGCCAATGCAGAGTGTGTGTGGCACATCGCACCTCCCCCAAAGCGCAGGATCCTAATCGTAGTC
  CCGGAGATCTTCCTACCCATCGAGGACGAGTGTGGTGATGTCCTGGTCATGAGAAAGAGTGCCTCGCCCA
  CGTCAGTCACCACCTATGAGACGTGCCAGACCTATGAGCGGCCCATCGCCTTTACCTCCCGTTCCCGCAA
  ACTCTGGATCCAGTTCAAATCCAACGAAGCCAACAGTGGCAAAGGGTTTCAAGTGCCCTATGTCACCTAT
  GATGAGGACTACCAGCAGCTAATAGAAGACATCGTGCGTGATGGGCGCTTATATGCCTCGGAGAACCATC
  AGGAGATTTTGAAAGACAAGAAGCTGATCAAGGCCCTCTTTGACGTGCTGGCACACCCACAAAACTACTT
  CAAATACACAGCCCAGGAGTCCAAGGAGATGTTCCCCCGGTCCTTTATCAAACTTCTGCGTTCCAAAGTG
  TCTCGGTTCCTGCGGCCCTACAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225502 representing NM_022723
  Red=Cloning site Green=Tags(s)
  
  MGAAAVRWHLYLLLALGARGRLVGGSGLPGAVDVDECSEGTDDCHIDAICQNTPKSYKCLCKPGYKGEGR
  QCEDIDECENDYYNGGCVHDCINIPGNYRCTCFDGFMLAHDGHNCLDVDECQDNNGGCQQICVNAMGSYE
  CQCHSGFFLSDNQHTCIHRSNEGMNCMNKDHGCAHICRETPKGGVACDCRPGFDLAQNQKDCTLTCNYGN
  GGCQHSCEDTDTGPMCGCHQKYALHADGRTCIETCAVNNGGCDRTCKDTATGVRCSCPVGFTLQPDGKTC
  KDINECLMNNGGCDHFCRNTVGSFECGCQKGHKLLTDERTCQDIDECSFERTCDHICINSPGSFQCLCRR
  GYTLYGTTHCGDVDECSMNNGSCEQGCVNTKGSYECVCPPGRRLHWNQKDCVEMNGCLSRSKASAQAQLS
  CGKVGGVENCFLSCLGHSLFMPDSESSYILSCGVPGLQGKTLPKRNGTSSSTGPGCSDAPTTPIRQKARF
  KIRDAKCHLQPRSQERAKDTLRHPLLDNCHVTFVTLKCDSSKKRRRGRKSPSKEVSHITAEFEVEMKVDE
  ASGTCEADCMRKRAEQSLQAAIKILRKSIGRNQFYVQVLGTEYEVAQRPAKALEGTGTCGIGQILQDGKC
  VPCAPGTYFSGDPGQCMPCVSGTYQDMEGQLSCTPCPSSEGLGLAGARNVSECGGQCSPGYFSADGFKPC
  QACPVGTYQPEPGRTGCFPCGGGLLTKHTGTASFQDCEAKVHCSPGHHYNTTTHRCIRCPVGTYQPEFGQ
  NHCISCPGNTSTDFDGSTNVTHCKNQHCGGELGDYTGYIESPNYPGDYPANAECVWHIAPPPKRRILIVV
  PEIFLPIEDECGDVLVMRKSASPTSVTTYETCQTYERPIAFTSRSRKLWIQFKSNEANSGKGFQVPYVTY
  DEDYQQLIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESKEMFPRSFIKLLRSKV
  SRFLRPYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022723
• ORF Size: 2964 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022723.3, NP_073560.2
• RefSeq Size: 7824 bp
• RefSeq ORF: 2967 bp
• Locus ID: 64706
• UniProt ID: Q6NZL8
• Cytogenetics: 15 E1
• Gene Summary: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.[UniProtKB/Swiss-Prot Function]