P3h3 (NM_013534) Mouse Untagged Clone

CAT#: MC221153

Leprel2 (untagged) - Mouse leprecan-like 2 (Leprel2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P3h3
• Synonyms: BC016431; Grcb; Leprel2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221153 representing NM_013534
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGGCTCCTGCGGTTGTTGCTTTTGCTGCTGCTGCCTCCCCCGGGGTCCCCCGAGCCTCCCGAGC
  CCCCGGGCCTGGCTCAGTTGTCACCGGGGTCGCCTCCCCAGGCCCCCGACTTGCTCTACGCGGACGGGCT
  GCGAGCCTACTCGGCCGGGGCTTGGGCGCCCGCAGTGGCGCTGCTGCGAGAGGCGCTGCGGAGCCGGGCG
  GCGCTGGGCCGCGCGCGCCAGGAGTGCGGGGCGAGCTGCGCGGCCGAGCCGGGCGCCGCGCTCCCCTCCC
  AGCTCCTCGGAGCCCCACATCCTGTCTCCGGGCCCGGGGTCTGGGAACCGCTGCTCCTGCGCGCCACGCT
  CCGCCGCGCCGAGTGCCTGACCCAGTGCGCGGTGCGGAGGCTGGGCCCGGGGGGCGCGGCGCGGCTCCGC
  GTGGGGAGCGCGCTGCGGGATGCCTTCCGCCGGCGGGAGCCTTACAACTACCTGCAGAGGGCCTACTACC
  AGTTGAAGAAGCTGGACCTGGCAGCTTCTGCAGCACACACCTTCTTTGTGGCAAACCCAACACACCTGCA
  GATGCGGGAAGACATGGCTAAGTACAGAAGGATGTCTGCAATCCGACCCCAGAGCTTCCGGGACCTGGTG
  ACGCCCCTATACTGGGCAGCTTATGACACTGGCCTGGAGCTTCTGGAGCAACGGGAGGCAGCACTGGCTC
  TACCCCAACTAGAGGAGGCCCTGCAGGGGAGCCTGGCCCACATGGAGAGCTGCCGTGCTGCCTGTGAGGG
  GCCTGAGGAGCACCAAGGGGCTGAAGAAGAGGGAGAAGGGAGCCAGGGAGGCCTGTATGAAGCCATTGCA
  GGACACTGGATACGGGTTCTGCAGTGCAGGCAGCACTGTGTGGCAGACACGGCCACCCGTCCTGGTCGCA
  GCTTCCCTGTCCAGGACTTCCTCCTCAGCCAGCTGAGACGGCTGCACGAGGCGTATGCTCAGGTGGGGAA
  CATGTCTCAGGCCATGGAAAATGTCCTGAGTGTCCTGCTCTTCTACCCAGAGGATGAGGCTGCCAAAAAG
  GCTCTGAACCAGTACCAAACTCAGTTGGGAGAGCCAAGACCTGACCTGGGACCACGAGAGGACATCCAGC
  GGTTCATCCTTCGCTCCCTCGGAGAGAAGAGACAGTTATATTACGCCATGGAGCATCTGGGCACTAGCTT
  CAAGGACCCGGATTCTTGGACCCCGGAGGCTCTCATCCCTAAGGCACTGAGAGAGAGGCTCAGAGAGGAT
  CAAGAGAAGAAGCCCTGGGACCATCAGCCTCCACAGCAGAAGCCCTTGGCTCACTGGAAGGATGCCCTTC
  TGATGGAGGGTGTGACCCTCACGCAGGACGCCCAGCAGCTGAATGGGTCGGAGCGAGCTGTCTTAGATGG
  GCTGCTAACTTCAGCCGAGTGTGGGGTTCTGCTGCAGCTGGCCAAGGATGCAGCACAGGCTGGAGCCAGG
  TCAGGCTACCGTGGCCGCCGCTCTCCTCATAGTCCTCATGAACGCTTTGAGGGGCTCACGGTGCTCAAGG
  CTGCTCAGCTGGCCCGGGCAGGGACTGTGGGCAGGCCAGGCGCTAAGCTGCTTCTGGAGGTAAGTGAGCG
  AGTGCGGACTTTGACCCAGGCCTACTTCTCCCCGGAACGGCCACTGCATCTTTCCTTCACCCACCTGGTG
  TGCCGAAGTGCCATAGAAGGTGAACAAGAGCAGCGTATGGACCTGAGTCACCCGGTTCATGCAGACAACT
  GTGTCCTGGACCCCGACACTGGCGAATGCTGGCGGGAGCCCCCAGCCTACACCTATCGAGACTATAGTGG
  ACTCCTCTACCTCAATGATGACTTCAAGGGAGGCGACCTGTTCTTCACGCAGCCCAACGCCCTCACTGTC
  ACGGCTCAGGTCCGTCCTCGGTGTGGGCGCCTTGTGGCCTTCAGCTCTGGTGGTGAGAATCCCCATGGTG
  TATGGGCTGTGACTCGGGGACGGCGCTGTGCCCTAGCACTGTGGCACACGTGGGCACCTGAGCACAGTGA
  ACAGGAGTGGACAGAAGCCAAAGAGCTGCTGCAGGAGGAAGAGGAGGAAGAAGAGGAGGAAGACATTCTC
  AGCAGAGACCCTTCCCCAGAACCCCCAAGTCACAAGCTTCAGCGAGTCCAGGAGAAAGCTGGGAAGCCCC
  GCCGGGTCCGGGTCCGAGAGGAACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013534
• Insert Size: 2199 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013534.4, NP_038562.2
• RefSeq Size: 2779 bp
• RefSeq ORF: 2199 bp
• Locus ID: 14789
• UniProt ID: Q8CG70
• Cytogenetics: 6 59.17 cM
• Gene Summary: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea (PubMed:28115524). Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:27119146, PubMed:28115524). Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases (PubMed:28115524).[UniProtKB/Swiss-Prot Function]