P3h3 (NM_013534) Mouse Tagged ORF Clone

CAT#: MG224833

Leprel2 (tGFP-tagged) - Mouse leprecan-like 2 (Leprel2), (10ug)

Product Images

Circular map for MG224833

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: P3h3
• Synonyms: BC016431; Grcb; Leprel2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224833 representing NM_013534
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGGCTCCTGCGGTTGTTGCTTTTGCTGCTGCTGCCTCCCCCGGGGTCCCCCGAGCCTCCCGAGC
  CCCCGGGCCTGGCTCAGTTGTCACCGGGGTCGCCTCCCCAGGCCCCCGACTTGCTCTACGCGGACGGGCT
  GCGAGCCTACTCGGCCGGGGCTTGGGCGCCCGCAGTGGCGCTGCTGCGAGAGGCGCTGCGGAGCCGGGCG
  GCGCTGGGCCGCGCGCGCCAGGAGTGCGGGGCGAGCTGCGCGGCCGAGCCGGGCGCCGCGCTCCCCTCCC
  AGCTCCTCGGAGCCCCACATCCTGTCTCCGGGCCCGGGGTCTGGGAACCGCTGCTCCTGCGCGCCACGCT
  CCGCCGCGCCGAGTGCCTGACCCAGTGCGCGGTGCGGAGGCTGGGCCCGGGGGGCGCGGCGCGGCTCCGC
  GTGGGGAGCGCGCTGCGGGATGCCTTCCGCCGGCGGGAGCCTTACAACTACCTGCAGAGGGCCTACTACC
  AGTTGAAGAAGCTGGACCTGGCAGCTTCTGCAGCACACACCTTCTTTGTGGCAAACCCAACACACCTGCA
  GATGCGGGAAGACATGGCTAAGTACAGAAGGATGTCTGCAATCCGACCCCAGAGCTTCCGGGACCTGGTG
  ACGCCCCTATACTGGGCAGCTTATGACACTGGCCTGGAGCTTCTGGAGCAACGGGAGGCAGCACTGGCTC
  TACCCCAACTAGAGGAGGCCCTGCAGGGGAGCCTGGCCCACATGGAGAGCTGCCGTGCTGCCTGTGAGGG
  GCCTGAGGAGCACCAAGGGGCTGAAGAAGAGGGAGAAGGGAGCCAGGGAGGCCTGTATGAAGCCATTGCA
  GGACACTGGATACGGGTTCTGCAGTGCAGGCAGCACTGTGTGGCAGACACGGCCACCCGTCCTGGTCGCA
  GCTTCCCTGTCCAGGACTTCCTCCTCAGCCAGCTGAGACGGCTGCACGAGGCGTATGCTCAGGTGGGGAA
  CATGTCTCAGGCCATGGAAAATGTCCTGAGTGTCCTGCTCTTCTACCCAGAGGATGAGGCTGCCAAAAAG
  GCTCTGAACCAGTACCAAACTCAGTTGGGAGAGCCAAGACCTGACCTGGGACCACGAGAGGACATCCAGC
  GGTTCATCCTTCGCTCCCTCGGAGAGAAGAGACAGTTATATTACGCCATGGAGCATCTGGGCACTAGCTT
  CAAGGACCCGGATTCTTGGACCCCGGAGGCTCTCATCCCTAAGGCACTGAGAGAGAGGCTCAGAGAGGAT
  CAAGAGAAGAAGCCCTGGGACCATCAGCCTCCACAGCAGAAGCCCTTGGCTCACTGGAAGGATGCCCTTC
  TGATGGAGGGTGTGACCCTCACGCAGGACGCCCAGCAGCTGAATGGGTCGGAGCGAGCTGTCTTAGATGG
  GCTGCTAACTTCAGCCGAGTGTGGGGTTCTGCTGCAGCTGGCCAAGGATGCAGCACAGGCTGGAGCCAGG
  TCAGGCTACCGTGGCCGCCGCTCTCCTCATAGTCCTCATGAACGCTTTGAGGGGCTCACGGTGCTCAAGG
  CTGCTCAGCTGGCCCGGGCAGGGACTGTGGGCAGGCCAGGCGCTAAGCTGCTTCTGGAGGTAAGTGAGCG
  AGTGCGGACTTTGACCCAGGCCTACTTCTCCCCGGAACGGCCACTGCATCTTTCCTTCACCCACCTGGTG
  TGCCGAAGTGCCATAGAAGGTGAACAAGAGCAGCGTATGGACCTGAGTCACCCGGTTCATGCAGACAACT
  GTGTCCTGGACCCCGACACTGGCGAATGCTGGCGGGAGCCCCCAGCCTACACCTATCGAGACTATAGTGG
  ACTCCTCTACCTCAATGATGACTTCAAGGGAGGCGACCTGTTCTTCACGCAGCCCAACGCCCTCACTGTC
  ACGGCTCAGGTCCGTCCTCGGTGTGGGCGCCTTGTGGCCTTCAGCTCTGGTGGTGAGAATCCCCATGGTG
  TATGGGCTGTGACTCGGGGACGGCGCTGTGCCCTAGCACTGTGGCACACGTGGGCACCTGAGCACAGTGA
  ACAGGAGTGGACAGAAGCCAAAGAGCTGCTGCAGGAGGAAGAGGAGGAAGAAGAGGAGGAAGACATTCTC
  AGCAGAGACCCTTCCCCAGAACCCCCAAGTCACAAGCTTCAGCGAGTCCAGGAGAAAGCTGGGAAGCCCC
  GCCGGGTCCGGGTCCGAGAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224833 representing NM_013534
  Red=Cloning site Green=Tags(s)
  
  MLRLLRLLLLLLLPPPGSPEPPEPPGLAQLSPGSPPQAPDLLYADGLRAYSAGAWAPAVALLREALRSRA
  ALGRARQECGASCAAEPGAALPSQLLGAPHPVSGPGVWEPLLLRATLRRAECLTQCAVRRLGPGGAARLR
  VGSALRDAFRRREPYNYLQRAYYQLKKLDLAASAAHTFFVANPTHLQMREDMAKYRRMSAIRPQSFRDLV
  TPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEGPEEHQGAEEEGEGSQGGLYEAIA
  GHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGNMSQAMENVLSVLLFYPEDEAAKK
  ALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLRED
  QEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGAR
  SGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLV
  CRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFKGGDLFFTQPNALTV
  TAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSEQEWTEAKELLQEEEEEEEEEDIL
  SRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013534
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013534.5
• RefSeq Size: 2779 bp
• RefSeq ORF: 2199 bp
• Locus ID: 14789
• UniProt ID: Q8CG70
• Cytogenetics: 6 59.17 cM
• Gene Summary: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea (PubMed:28115524). Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:27119146, PubMed:28115524). Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases (PubMed:28115524).[UniProtKB/Swiss-Prot Function]