Dnm1l (NM_152816) Mouse Untagged Clone

CAT#: MC221017

Dnm1l (untagged) - Mouse dynamin 1-like (Dnm1l), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnm1l
• Synonyms: 6330417M19Rik; AI450666; Dlp1; Dnm; Dnmlp1; Dr; Drp1; pyt; python
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221017 representing NM_152816
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC
  AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
  CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT
  TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGACCCTGCCACATGGAAAAACTCAAGACACC
  TTTCCAAAGGAGTTGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAAAAACAAGCTTTACACAGATTT
  TGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAGAATTTCAGGAAATAATAAGGGGGTAAGCCCT
  GAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTCAACCTGACACTTGTGGATTTACCGGGAATGA
  CCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGCTTCAGATCAGAGAACTTATTCTTCGGTTCAT
  CAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGCAAATACAGATATGGCAACATCAGAAGCACTC
  AAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACTCTAGCTGTAATCACTAAACTTGATCTCATGG
  ATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAGTCAAGCTTGGAATAATTGGAGT
  AGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCT
  TTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAATGGAACAAAGTATCTTGCTAGGACCCTGAATA
  GGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGCTGAAAACAAGAATAAATGTCTTAGCTGCTCA
  GTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGATGATAAAAGTGCTACTTTACTCCAGCTTATT
  ACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGAACCGCAAAGTACATTGAAACTTCTGAGCTAT
  GCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTTTCGGGCGAACCTTAGAATCTGTGGACCCACT
  AGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAGAAATGCTACTGGCCCCCGTCCTGCTTTATTT
  GTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAGATTAAGCGTCTAGAAGAGCCCAGCCTACGGT
  GTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTCAGCATTGTAGCAATTACAGCACACAGGAATT
  GTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGTAGTGACCTGTCTTCTTCGTAAAAGGTTGCCC
  GTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAGCTAGCGTATATCAACACAAAACACCCCGACT
  TTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGGAACAAAGAAGAAACAGGCTAGCAAGAGAGCT
  GCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGGTGGTGGGATTGGAGACGGTGGTCAGGAACCA
  ACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTGAAGAAAAATCAA
  AACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCCATGCTGTCAATTTGCTAGATGTGCCAGTTCC
  AGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTTATCAAATCATAT
  TTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCAAAGGCAGTAATGCATTTTTTGGTTAATCATG
  TGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGTATAAGTCATCCTTATTAGATGACCTTCTGAC
  TGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGCGGATATGCTGAAGGCATTACAAGGAGCCAGT
  CAAATTATTGCTGAAATCCGAGAGACTCATCTTTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_152816
• Insert Size: 2139 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152816.3, NP_690029.2
• RefSeq Size: 4073 bp
• RefSeq ORF: 2139 bp
• Locus ID: 74006
• UniProt ID: Q8K1M6
• Cytogenetics: 16 A2
• Gene Summary: This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (1) has multiple differences in the coding region but maintains the reading frame, compared to variant 6. The encoded isoform (a) is shorter than isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.