Dnm1l (NM_152816) Mouse Tagged ORF Clone

CAT#: MG225302

Dnm1l (tGFP-tagged) - Mouse dynamin 1-like (Dnm1l) transcript variant 1, (10ug)

Product Images

Circular map for MG225302

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnm1l
• Synonyms: 6330417M19Rik; AI450666; Dlp1; Dnm; Dnmlp1; Dr; Drp1; pyt; python
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225302 representing NM_152816
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC
  AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
  CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT
  TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGACCCTGCCACATGGAAAAACTCAAGACACC
  TTTCCAAAGGAGTTGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAAAAACAAGCTTTACACAGATTT
  TGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAGAATTTCAGGAAATAATAAGGGGGTAAGCCCT
  GAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTCAACCTGACACTTGTGGATTTACCGGGAATGA
  CCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGCTTCAGATCAGAGAACTTATTCTTCGGTTCAT
  CAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGCAAATACAGATATGGCAACATCAGAAGCACTC
  AAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACTCTAGCTGTAATCACTAAACTTGATCTCATGG
  ATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAGTCAAGCTTGGAATAATTGGAGT
  AGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCT
  TTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAATGGAACAAAGTATCTTGCTAGGACCCTGAATA
  GGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGCTGAAAACAAGAATAAATGTCTTAGCTGCTCA
  GTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGATGATAAAAGTGCTACTTTACTCCAGCTTATT
  ACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGAACCGCAAAGTACATTGAAACTTCTGAGCTAT
  GCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTTTCGGGCGAACCTTAGAATCTGTGGACCCACT
  AGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAGAAATGCTACTGGCCCCCGTCCTGCTTTATTT
  GTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAGATTAAGCGTCTAGAAGAGCCCAGCCTACGGT
  GTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTCAGCATTGTAGCAATTACAGCACACAGGAATT
  GTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGTAGTGACCTGTCTTCTTCGTAAAAGGTTGCCC
  GTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAGCTAGCGTATATCAACACAAAACACCCCGACT
  TTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGGAACAAAGAAGAAACAGGCTAGCAAGAGAGCT
  GCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGGTGGTGGGATTGGAGACGGTGGTCAGGAACCA
  ACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTGAAGAAAAATCAA
  AACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCCATGCTGTCAATTTGCTAGATGTGCCAGTTCC
  AGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTTATCAAATCATAT
  TTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCAAAGGCAGTAATGCATTTTTTGGTTAATCATG
  TGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGTATAAGTCATCCTTATTAGATGACCTTCTGAC
  TGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGCGGATATGCTGAAGGCATTACAAGGAGCCAGT
  CAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225302 representing NM_152816
  Red=Cloning site Green=Tags(s)
  
  MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV
  SPEDKRKTTGEENDPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSP
  EPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEAL
  KISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYA
  FLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLI
  TKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALF
  VPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLP
  VTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAGSRDKVPSAGGGIGDGGQEP
  TTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSY
  FLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGAS
  QIIAEIRETHLW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152816
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152816.3, NP_690029.2
• RefSeq Size: 4078 bp
• RefSeq ORF: 2139 bp
• Locus ID: 74006
• UniProt ID: Q8K1M6
• Cytogenetics: 16 A2
• Gene Summary: This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]