Nox4 (NM_015760) Mouse Untagged Clone

CAT#: MC219182

Nox4 (untagged) - Mouse NADPH oxidase 4 (Nox4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nox4
• Synonyms: AI648021
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015760, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGTGTCCTGGAGGAGCTGGCTGGCCAACGAAGGGGTTAAACACCTCTGCCTGCTCATTTGGCTGT
  CCCTAAACGTTCTACTTTTCTGGAAAACCTTCCTGCTGTACAACCAAGGGCCAGAATACTACTACATTCA
  CCAAATGTTGGGCCTAGGATTGTGTTTAAGCAGAGCATCTGCATCTGTCCTGAACCTCAACTGCAGCCTC
  ATCCTTTTACCTATGTGCCGGACAGTCCTGGCTTATCTTCGAGGATCACAGAAGGTCCCTAGCAGGAGAA
  CAAGAAGATTGTTGGATAAAAGCAAGACTCTACACATCACATGTGGTGTAACTATCTGTATTTTCTCAGG
  TGTGCATGTAGCCGCCCACTTGGTGAATGCCCTCAACTTTTCAGTGAACTACAGTGAAGATTTCCTTGAA
  CTGAATGCAGCAAGATACCAGAATGAGGATCCCAGAAAGCTTCTCTTCACAACCATTCCTGGTCTGACGG
  GTGTCTGCATGGTGGTGGTATTGTTCCTCATGGTTACAGCTTCTACCTACGCAATAAGAGTTTCTAATTA
  TGATATCTTCTGGTATACTCACAACCTCTTCTTTGTCTTCTACATGCTGCTGCTGTTGCATGTTTCAGGT
  GGTTTGTTGAAGTATCAGACAAATGTAGACACTCACCCTCCTGGCTGCATTAGTCTTAACCAGACATCAT
  CCCAGAATATGTCCATACCAGACTACGTCTCAGAACATTTTCATGGATCTTTGCCTCGAGGGTTTTCAAA
  ATTAGAAGATCGTTACCAGAAAACACTTGTGAAGATTTGCCTGGAAGAACCCAAGTTCCAAGCTCATTTC
  CCACAGACCTGGATTTGGATTTCTGGACCTTTGTGCCTTTATTGTGCGGAGAGACTTTACCGATGCATCA
  GGAGCAACAAACCTGTCACCATCATCTCAGTCATCAATCATCCCTCTGATGTAATGGAACTCCGTATGAT
  CAAAGAAAACTTTAAAGCAAGACCTGGTCAGTATATTATTCTCCATTGCCCCAGTGTATCAGCATTAGAA
  AACCACCCATTTACTCTCACAATGTGTCCTACTGAAACCAAAGCAACATTTGGTGTCCACTTTAAAGTAG
  TAGGAGACTGGACAGAACGATTCCGGGATTTGCTACTGCCTCCATCAAGTCAAGACTCTGAGATTCTGCC
  CTTCATTCACTCTAGAAATTACCCTAAGTTATACATTGATGGTCCATTTGGAAGCCCATTTGAGGAGTCA
  CTGAACTATGAAGTTAGTCTCTGTGTGGCTGGAGGCATTGGAGTCACTCCATTTGCATCGATACTAAACA
  CTCTACTGGATGACTGGAAACCATACAAGTTAAGAAGACTGTACTTTATCTGGGTGTGCAGAGACATCCA
  ATCATTCCAGTGGTTTGCAGATTTACTCTGTGTGTTGCATAACAAGTTTTGGCAAGAAAACAGACCTGAC
  TTTGTGAACATCCAGCTGTACCTCAGTCAAACAGATGGGATTCAGAAGATAATTGGAGAAAAATATCACA
  CACTGAATTCGAGACTTTTCATTGGACGTCCTCGGTGGAAACTTTTATTTGATGAAATAGCAAAATGTAA
  CAGAGGGAAAACAGTTGGAGTTTTCTGCTGTGGACCCAGTTCTATTTCCAAGACTCTTCATAGTTTGAGT
  AACCGGAACAACTCATATGGGACAAAATTTGAATACAATAAAGAATCCTTCAGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015760
• Insert Size: 1737 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138918, AAI38919
• RefSeq Size: 2343 bp
• RefSeq ORF: 1737 bp
• Locus ID: 50490
• UniProt ID: Q9JHI8
• Cytogenetics: 7 D3
• Gene Summary: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).