Gga2 (BC057377) Mouse Untagged Clone

CAT#: MC217904

Gga2 (untagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 2 (cDNA clone MGC:67474 IMAGE:5698559),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gga2
• Synonyms: 1200007E24Rik; mKIAA1080
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057377
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCATGTACCGAAGGCCAGGTCATGCCCTGCCCGACCAGCAGGCCCTGCAGGTTGTGTATGAAA
  GATGTGAGAAGCTGAGGCCCACCCTGTTCCGGCTTGCAAGCGACACCACTGATGACGACGATGCTCTGGC
  GGAGATTCTTCAAGCAAATGACCTTCTCACACAGGGGGTTCGGCTGTACAAACAGGTGGTGGAGGGCCGA
  GTTAGTGCTGGGAACGCAGTGCCTGCCGCAGTGGGAGCCATTCCCGCCCCCAGAGCATTCCCGAATCCAG
  AACCCTGTGGGTTGAACTGTCCACTCATTGACCTGGAGACGCCATCTTTGCTTCATCAGGACCTGGCAGC
  CTTAGGAATCAACGATGTTCCCACTAGGAACCAGGTTGTTATTCCGAGTTGCTGTAATGACAAGAAGCAG
  CCGGGTGCCATCACACTCATGGGGGGTGGAATCCAGAGTCTTTCTGCAGACCGGAACCTGCTGGATCTCT
  TCTCGCCACAGCCATCTCCAGGACTGAATTATGTTCCACAAAAAAGCATCCCCAAGGAGGTGCCACCAGG
  TACCAAGGCCTCTCCAGGTTGGTCCTGGGAGGCTGGGCCATTGGCTTCTTCCACAGCCTCACAGAATACA
  CCTCTGGCTCATGTGTTTGTCCCTTTGGAGTCTGTGAAACCCAGCAGCTTGCCGCCCATTGTCGTGTATG
  ACCGGAATGGCTTCCGAATTCTGCTGCACTTCTCCCAGACTGGGGCCCCTGGCCACCCGGATGTGAAGGT
  GTTGTTGTTGACCATGATGAGCACTGCCACCCAACCTGTCTGGGATGTCATGTTTCAGGTGGCTGTCCCA
  AAGTCAATGCGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTCCCTGCATTCAGTCCTTTGATGCCTC
  CAGCAGTGATATCTCAGACGCTGTTGCTCGACAACCCACACAAAGAACCTATCCGGTTGCGGTATAAGCT
  GACTTTCAACCAAGGTGGGCAGCCGTTCAGTGAAGTAGGAGAGGTGAAAGACTTTCCAGACCTGGCTGTC
  TTGAGCACCGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057377
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057377, AAH57377
• RefSeq Size: 2370 bp
• RefSeq ORF: 1064 bp
• Locus ID: 74105
• Cytogenetics: 7 F2
• Gene Summary: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.[UniProtKB/Swiss-Prot Function]