Gga2 (BC057377) Mouse Tagged ORF Clone

CAT#: MG205395

Gga2 (tGFP-tagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 2 (cDNA clone MGC:67474 IMAGE:5698559)

Product Images

Circular map for MG205395

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gga2
• Synonyms: 1200007E24Rik; mKIAA1080
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205395 representing BC057377
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCATGTACCGAAGGCCAGGTCATGCCCTGCCCGACCAGCAGGCCCTGCAGGTTGTGTATGAAA
  GATGTGAGAAGCTGAGGCCCACCCTGTTCCGGCTTGCAAGCGACACCACTGATGACGACGATGCTCTGGC
  GGAGATTCTTCAAGCAAATGACCTTCTCACACAGGGGGTTCGGCTGTACAAACAGGTGGTGGAGGGCCGA
  GTTAGTGCTGGGAACGCAGTGCCTGCCGCAGTGGGAGCCATTCCCGCCCCCAGAGCATTCCCGAATCCAG
  AACCCTGTGGGTTGAACTGTCCACTCATTGACCTGGAGACGCCATCTTTGCTTCATCAGGACCTGGCAGC
  CTTAGGAATCAACGATGTTCCCACTAGGAACCAGGTTGTTATTCCGAGTTGCTGTAATGACAAGAAGCAG
  CCGGGTGCCATCACACTCATGGGGGGTGGAATCCAGAGTCTTTCTGCAGACCGGAACCTGCTGGATCTCT
  TCTCGCCACAGCCATCTCCAGGACTGAATTATGTTCCACAAAAAAGCATCCCCAAGGAGGTGCCACCAGG
  TACCAAGGCCTCTCCAGGTTGGTCCTGGGAGGCTGGGCCATTGGCTTCTTCCACAGCCTCACAGAATACA
  CCTCTGGCTCATGTGTTTGTCCCTTTGGAGTCTGTGAAACCCAGCAGCTTGCCGCCCATTGTCGTGTATG
  ACCGGAATGGCTTCCGAATTCTGCTGCACTTCTCCCAGACTGGGGCCCCTGGCCACCCGGATGTGAAGGT
  GTTGTTGTTGACCATGATGAGCACTGCCACCCAACCTGTCTGGGATGTCATGTTTCAGGTGGCTGTCCCA
  AAGTCAATGCGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTCCCTGCATTCAGTCCTTTGATGCCTC
  CAGCAGTGATATCTCAGACGCTGTTGCTCGACAACCCACACAAAGAACCTATCCGGTTGCGGTATAAGCT
  GACTTTCAACCAAGGTGGGCAGCCGTTCAGTGAAGTAGGAGAGGTGAAAGACTTTCCAGACCTGGCTGTC
  TTGAGCACCGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205395 representing BC057377
  Red=Cloning site Green=Tags(s)
  
  MLSMYRRPGHALPDQQALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQANDLLTQGVRLYKQVVEGR
  VSAGNAVPAAVGAIPAPRAFPNPEPCGLNCPLIDLETPSLLHQDLAALGINDVPTRNQVVIPSCCNDKKQ
  PGAITLMGGGIQSLSADRNLLDLFSPQPSPGLNYVPQKSIPKEVPPGTKASPGWSWEAGPLASSTASQNT
  PLAHVFVPLESVKPSSLPPIVVYDRNGFRILLHFSQTGAPGHPDVKVLLLTMMSTATQPVWDVMFQVAVP
  KSMRVKLQPASSSKLPAFSPLMPPAVISQTLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGEVKDFPDLAV
  LSTA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057377
• ORF Size: 1064 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057377, AAH57377
• RefSeq Size: 2370 bp
• RefSeq ORF: 1064 bp
• Locus ID: 74105
• Cytogenetics: 7 F2
• Gene Summary: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.[UniProtKB/Swiss-Prot Function]