Cyr61 (NM_010516) Mouse Untagged Clone

CAT#: MC208761

Cyr61 (untagged) - Mouse cysteine rich protein 61 (Cyr61), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyr61
• Synonyms: AI325051; CCN1; Igfbp10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208761 representing NM_010516
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCAGCACCTTCAGGACGCTCGCTGTCGCCGTCACCCTTCTCCACTTGACCAGACTGGCGCTCT
  CCACCTGCCCCGCCGCCTGCCACTGCCCTCTGGAGGCACCCAAGTGCGCCCCGGGAGTCGGGTTGGTCCG
  GGACGGCTGCGGCTGCTGTAAGGTCTGCGCTAAACAACTCAACGAGGACTGCAGCAAAACTCAGCCCTGC
  GACCACACCAAGGGGTTGGAATGCAATTTCGGCGCCAGCTCCACCGCTCTGAAAGGGATCTGCAGAGCTC
  AGTCAGAAGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGCTTCCAGCCCAACTG
  TAAACACCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTGTCTCTC
  CCCAATCTGGGCTGTCCCAACCCCCGGCTGGTGAAAGTCAGCGGGCAGTGCTGTGAAGAGTGGGTTTGTG
  ATGAAGACAGCATTAAGGACTCCCTGGACGACCAGGATGACCTCCTCGGACTCGATGCCTCGGAGGTGGA
  GTTAACGAGAAACAATGAGTTAATCGCAATTGGAAAAGGCAGCTCACTGAAGAGGCTTCCTGTCTTTGGC
  ACCGAACCGCGAGTTCTTTTCAACCCTCTGCACGCCCATGGCCAGAAATGCATCGTTCAGACCACGTCTT
  GGTCCCAGTGCTCCAAGAGCTGCGGAACTGGCATCTCCACACGAGTTACCAATGACAACCCAGAGTGCCG
  CCTGGTGAAAGAGACCCGGATCTGTGAAGTGCGTCCTTGTGGACAACCAGTGTACAGCAGCCTAAAAAAG
  GGCAAGAAATGCAGCAAGACCAAGAAATCCCCAGAACCAGTCAGATTTACTTATGCAGGATGCTCCAGTG
  TCAAGAAATACCGGCCCAAATACTGCGGCTCCTGCGTAGATGGCCGGTGCTGCACACCTCTGCAGACCAG
  AACTGTGAAGATGCGGTTCCGATGCGAAGATGGAGAGATGTTTTCCAAGAATGTCATGATGATCCAGTCC
  TGCAAATGTAACTACAACTGCCCGCATCCCAACGAGGCATCGTTCCGACTGTACAGCCTATTCAATGACA
  TCCACAAGTTCAGGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010516
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066019, AAH66019
• RefSeq Size: 2045 bp
• RefSeq ORF: 1140 bp
• Locus ID: 16007
• UniProt ID: P18406
• Cytogenetics: 3 70.18 cM
• Gene Summary: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CCN1-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).[UniProtKB/Swiss-Prot Function]