Cyr61 (NM_010516) Mouse Tagged ORF Clone

CAT#: MG221828

Cyr61 (tGFP-tagged) - Mouse cysteine rich protein 61 (Cyr61), (10ug)

Product Images

Circular map for MG221828

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyr61
• Synonyms: AI325051; CCN1; Igfbp10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221828 representing NM_010516
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCAGCACCTTCAGGACGCTCGCTGTCGCCGTCACCCTTCTCCACTTGACCAGACTGGCGCTCT
  CCACCTGCCCCGCCGCCTGCCACTGCCCTCTGGAGGCACCCAAGTGCGCCCCGGGAGTCGGGTTGGTCCG
  GGACGGCTGCGGCTGCTGTAAGGTCTGCGCTAAACAACTCAACGAGGACTGCAGCAAAACTCAGCCCTGC
  GACCACACCAAGGGGTTGGAATGCAATTTCGGCGCCAGCTCCACCGCTCTGAAAGGGATCTGCAGAGCTC
  AGTCAGAAGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGCTTCCAGCCCAACTG
  TAAACACCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTGTCTCTC
  CCCAATCTGGGCTGTCCCAACCCCCGGCTGGTGAAAGTCAGCGGGCAGTGCTGTGAAGAGTGGGTTTGTG
  ATGAAGACAGCATTAAGGACTCCCTGGACGACCAGGATGACCTCCTCGGACTCGATGCCTCGGAGGTGGA
  GTTAACGAGAAACAATGAGTTAATCGCAATTGGAAAAGGCAGCTCACTGAAGAGGCTTCCTGTCTTTGGC
  ACCGAACCGCGAGTTCTTTTCAACCCTCTGCACGCCCATGGCCAGAAATGCATCGTTCAGACCACGTCTT
  GGTCCCAGTGCTCCAAGAGCTGCGGAACTGGCATCTCCACACGAGTTACCAATGACAACCCAGAGTGCCG
  CCTGGTGAAAGAGACCCGGATCTGTGAAGTGCGTCCTTGTGGACAACCAGTGTACAGCAGCCTAAAAAAG
  GGCAAGAAATGCAGCAAGACCAAGAAATCCCCAGAACCAGTCAGATTTACTTATGCAGGATGCTCCAGTG
  TCAAGAAATACCGGCCCAAATACTGCGGCTCCTGCGTAGATGGCCGGTGCTGCACACCTCTGCAGACCAG
  AACTGTGAAGATGCGGTTCCGATGCGAAGATGGAGAGATGTTTTCCAAGAATGTCATGATGATCCAGTCC
  TGCAAATGTAACTACAACTGCCCGCATCCCAACGAGGCATCGTTCCGACTGTACAGCCTATTCAATGACA
  TCCACAAGTTCAGGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221828 representing NM_010516
  Red=Cloning site Green=Tags(s)
  
  MSSSTFRTLAVAVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPC
  DHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSL
  PNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFG
  TEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKK
  GKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQS
  CKCNYNCPHPNEASFRLYSLFNDIHKFRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010516
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010516.2, NP_034646.1
• RefSeq Size: 2028 bp
• RefSeq ORF: 1140 bp
• Locus ID: 16007
• UniProt ID: P18406
• Cytogenetics: 3 70.18 cM
• Gene Summary: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CCN1-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).[UniProtKB/Swiss-Prot Function]