Sell (NM_011346) Mouse Untagged Clone

CAT#: MC207393

Sell (untagged) - Mouse selectin, lymphocyte (Sell), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sell
• Synonyms: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207393 representing NM_011346
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTCCATGGAGATGTGAGGGTACTTACTGGGGCTCGAGGAACATCCTGAAGCTGTGGGTCTGGA
  CACTGCTCTGTTGTGACTTCCTGATACACCATGGAACTCACTGTTGGACTTACCATTATTCTGAAAAGCC
  CATGAACTGGGAAAATGCTAGAAAGTTCTGCAAGCAAAATTACACAGATTTAGTCGCCATACAAAACAAG
  AGAGAAATTGAGTATTTAGAGAATACATTGCCCAAAAGCCCTTATTACTACTGGATAGGAATCAGGAAAA
  TTGGGAAAATGTGGACATGGGTGGGAACCAACAAAACTCTCACTAAAGAAGCAGAGAACTGGGGTGCTGG
  GGAGCCCAACAACAAGAAGTCCAAGGAGGACTGTGTGGAGATCTATATCAAGAGGGAACGAGACTCTGGG
  AAATGGAACGATGACGCCTGTCACAAACGAAAGGCAGCTCTCTGCTACACAGCCTCTTGCCAGCCAGGGT
  CTTGCAATGGCCGTGGAGAATGTGTGGAAACTATCAACAATCACACGTGCATCTGTGATGCAGGGTATTA
  CGGGCCCCAGTGTCAGTATGTGGTCCAGTGTGAGCCTTTGGAGGCCCCTGAGTTGGGTACCATGGACTGC
  ATCCACCCCTTGGGAAACTTCAGCTTCCAGTCCAAGTGTGCTTTCAACTGTTCTGAGGGAAGAGAGCTAC
  TTGGGACTGCAGAAACACAGTGTGGAGCATCTGGAAACTGGTCATCTCCAGAGCCAATCTGCCAAGTGGT
  CCAGTGTGAGCCTTTGGAGGCCCCTGAGTTGGGTACCATGGACTGCATCCACCCCTTGGGAAACTTCAGC
  TTCCAGTCCAAGTGTGCTTTCAACTGTTCTGAGGGAAGAGAGCTACTTGGGACTGCAGAAACACAGTGTG
  GAGCATCTGGAAACTGGTCATCTCCAGAGCCAATCTGCCAAGAGACAAACAGAAGTTTCTCAAAGATCAA
  AGAAGGTGACTACAACCCCCTCTTCATTCCTGTAGCCGTCATGGTCACCGCATTCTCGGGGCTGGCATTT
  CTCATTTGGCTGGCAAGGCGGTTAAAAAAAGGCAAGAAATCTCAAGAAAGGATGGATGATCCATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011346
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052681, AAH52681
• RefSeq Size: 2341 bp
• RefSeq ORF: 1119 bp
• Locus ID: 20343
• UniProt ID: P18337
• Cytogenetics: 1 71.37 cM
• Gene Summary: Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed:1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).