Sell Rabbit Monoclonal Antibody [Clone ID: MEL-14]

CAT#: TA386018

Recombinant Anti-CD62L (Clone MEL-14)

Product Images

Flow-cytometry using anti-CD62L antibody MEL-14 (TA386018) Mouse lymphocytes were stained with an anti-CD44 control (TA386028, panel A) or the rabbit-chimeric version of MEL-14 (TA386018, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

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  Flow-cytometry using the anti-CD62L (L-selectin) antibody MEL-14 (TA386018) Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (TA386018, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: MEL-14
• Applications: Bl, FC, IHC, IP
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: C3H/eb mouse B lymphoma 38C-13.
• Specificity: This antibody binds to mouse CD62L the mouse peripheral lymph node homing receptor, which is a lectin-like cell surface protein rapidly down-regulated upon cell activation with phorbol 12-myristate 13-acetate.
  
  This antibody detects mouse CD62L and has been reported to inhibit the interaction of neutrophils with endothelial cells in vitro and inhibit the migration of neutrophils from the blood into sites of acute inflammation in the skin (Lewinsohn et al. 1987).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: selectin, lymphocyte
• Database Link:
  Entrez Gene 20343 Mouse
  UniProt ID P18337
• Synonyms: CD62L; gp90-MEL; hLHRc; LAM-1; LAM1; LECAM1; Leu-8; LEU8; LNHR; LSEL; Lyam-1; LYAM1; PLNHR; TQ1
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.