CA 15-3 ELISA Kit

CAT#: EA101114

CA 15-3 ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human CA 15-3
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The CA 15-3 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a mouse monoclonal antibody directed towards a unique antigenic site of the CA15-3 molecule. An aliquot of sample containing endogenous CA15-3 is incubated in the coated well with enzyme conjugate, which is an anti-CA15-3 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA15-3 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA15-3 in the sample.