Human Nidogen-1/Entactin/NID-1 ELISA Kit

CAT#: EA100543

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Human Nidogen-1/Entactin/NID-1 ELISA Kit

USD 534.00

3 Weeks

    • 1 x 96 wells

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Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Nidogen-1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates and serum.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Nidogen-1
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 96-well plate precoated with anti- human Nidogen-1 antibody 1
  • Lyophilized recombinant human Nidogen-1 standard 10ng/tubex2
  • Biotinylated anti- human Nidogen-1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.
Gene Symbol NID1
Gene ID 4811
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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