HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/02]

CAT#: SM3053P

HLAE (HLA-E) mouse monoclonal antibody, clone MEM-E/02, Purified

Product Images

Immunohistochemistry: HLA-E antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: MEM-E/02
• Applications: IHC, WB
• Recommended Dilution: Western blotting.
  Immunohistochemistry on Paraffin Sections: 5-10 µg/ml.
  Heat antigen retrieval (Sodium Citrate).
  Positive Control: Human placenta.
  Incubation Time: 1 hour at RT (detection DAB).
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human HLA-E denatured heavy chain
• Specificity: The antibody specifically reacts with denaturated heavy chain of Human HLA-E.
• Formulation: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein-A.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, E
• Database Link:
  Entrez Gene 3133 Human
  UniProt ID P13747
• Background: HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
  This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
• Synonyms: HLA-6.2, HLAE, MHC class I antigen E