CD43 / Leukosialin Mouse Monoclonal Antibody [Clone ID: W3/13HLK]

CAT#: SM271BX

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CD43 / Leukosialin mouse monoclonal antibody, clone W3/13HLK, Biotin



Get 29% off any Over-Expression Cell Lysate, a validated WB control, with any Ab purchase. Use code “OEL29“. View details.

USD 500.00


Availability*
2 Weeks

Size
    • 500 ug


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Specifications

Product Data
Clone Name W3/13HLK
Applications FC
Recommended Dilution Flow Cytometry.
Tissue Distribution by Flow Cytometry Analysis:
Rat Strain: Wister Rat.
Cell Concentration: 1x10e6 cells per test.
Antibody Concentration Used: 2.0 μg/106 cells.
Secondary Antibody: Streptavidin FITC.
Cell Source: Percentage of cells stained above control
Thymus: 69%
Spleen: 40%
Reactivity Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Rat thymocyte membrane preparation.
Specificity This CD43 monoclonal antibody recognizes a monomorphic determinant expressed on Rat thymocytes, polymorphonuclear cells, plasma cells and stem cells, but not B lymphocytes or pre-B cells (1,3).
This antibody is useful for labelling T but not B lymphocytes and in studies on stem cells since pre-B cells are not labelled while the multipotential stem cell is. It may also be used in analysis of NK cells (5) and in molecular studies of the sialoglycoprotein which it recognizes.
Formulation PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: Biotin
State: Liquid purified IgG fraction
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Stability Shelf life: one year from despatch.
Background The antigen is a heavily glycosylated glycoprotein of apparent molecular weight 95 kDa and has a high content of Olinked carbohydrate structures (3). This major glycoprotein of thymocytes and T lymphocytes is referred to by several names including leukocyte sialoglycoprotein and leukosialin. The carbohydrate structures of leukosialin account for approximately 60% of its weight (2). On thymocytes, this glycoprotein is the main target for binding of peanut lectin (4).
Synonyms Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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