TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]
TLR4 mouse monoclonal antibody, clone HTA125, PE
|Recommended Dilution||Flow Cytometry: Use 10 µl of neat antibody to label 106 cells or 100 µl whole blood.|
|Reactivity||Canine, Guinea Pig, Human, Monkey, Porcine|
|Immunogen||Ba/F3 cell line expressing TLR4 (CD284).
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
|Specificity||This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen.
This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
State: Lyophilized purified IgG fraction from Tissue Culture Supernatant
Stabilizer: 1% BSA and 5% Sucrose
Preservative: 0.09% Sodium Azide
Label: R. Phycoerythrin (RPE)
|Reconstitution Method||Restore with 1.0 ml distilled water.|
|Purification||Affinity Chromatography on Protein G|
Prior to and following reconstitution store the antibody at 2-8°C.
|Stability||Shelf life: one year from despatch.|
|Background||TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.|
|Synonyms||Toll-like receptor 4|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway|