E2F1 Mouse Monoclonal Antibody [Clone ID: KH95]

E2F1 mouse monoclonal antibody, clone KH95, Purified

USD 935.00

3 Days

    • 1 ml

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Product Data
Clone Name KH95
Applications IF, IHC, IP, WB
Recommended Dilution Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABC method for 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Recommended Positive Control: Tonsil, Breast carcinoma.
Reactivity Human, Mouse
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen BALB/C mice were immunized with a recombinant Human E2F-1 protein.
Specificity This antibody reacts with a protein of 60 kD.
Cellular Localization: Nuclear.
Formulation State: Purified
State: Purified Ascites with Sodium Azide as preservative.
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.
Synonyms E2F-1, RBBP3, PBR3
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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