E2F1 Mouse Monoclonal Antibody [Clone ID: KH95]
E2F1 mouse monoclonal antibody, clone KH95, Purified
|Applications||IF, IHC, IP, WB|
|Recommended Dilution||Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABC method for 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Recommended Positive Control: Tonsil, Breast carcinoma.
|Immunogen||BALB/C mice were immunized with a recombinant Human E2F-1 protein.|
|Specificity||This antibody reacts with a protein of 60 kD.
Cellular Localization: Nuclear.
State: Purified Ascites with Sodium Azide as preservative.
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.|
|Synonyms||E2F-1, RBBP3, PBR3|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.