CD283 / TLR3 (LRR20) Mouse Monoclonal Antibody [Clone ID: 1210F1 / TLR3.2]
CD283 / TLR3 (LRR20) mouse monoclonal antibody, clone 1210F1 / TLR3.2
|Clone Name||1210F1 / TLR3.2|
|Applications||FC, IF, IP, WB|
|Recommended Dilution||FACS intracellular, ImmunoFluorescence, Immunoprecipitation, Western Blot.
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
|Immunogen||HEK-293 stably transfected with huTLR3+rhu TLR3 ECD (R&D)|
Recognizez Mouse TLR3 (WB).
|Formulation||Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
Label: Alexa Fluor 546
|Purification||QMA Hyper D ion exchange chromatography|
|Conjugation||Alexa Fluor 546|
|Background||The Toll-like receptor (TLR) family are considered as key for sensing microbial infections and triggering inflammatory and innate responses. 3 novel mAbs were generated against TLR3 that support the emergence of an alternative model for TLR3 biology: TLR3 full-length (TLR3FL, 130KDa) corresponds to the TLR3 present in ER, which in resting cells is steadily converted within the Golgi into fully glycosylated TLR3 (TLR3FL+, 135KDa) and then rapidly cleaved by cathepsins in the endosome/lysosome into 2 long-lived associated fragments (72 KDa and 60KDa). (Toscano F et al, J. Immunol, 2013, 190: 764–773).|
|Synonyms||Toll-like receptor 3|