CALCA Mouse Monoclonal Antibody [Clone ID: OTI2D12]

CAT#: CF190011

Carrier-free (glycerol/BSA-free) CALCA mouse monoclonal antibody, clone OTI2D12 (formerly 2D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCA (RC214594, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCA.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CALCA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190011)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CALCA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190011)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CALCA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190011)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CALCA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190011)

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Specifications

Product Data

• Clone Name: OTI2D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CACLA ( NP_001732). The exact sequence is proprietary.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.