POLR2A (794-822) Mouse Monoclonal Antibody [Clone ID: ARNA-3]

CAT#: BM5528

POLR2A (794-822) mouse monoclonal antibody, clone ARNA-3, Supernatant

Specifications

Product Data

• Clone Name: ARNA-3
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting (Western blot).
  Immunohistochemistry (1/100).
  Suitable for Cell Culture and Frozen Tissue.
  Recommended Fixation conditions for Cells: Incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min.
  Incubation time: 1 h at RT for Immunohistochemistry.
• Reactivities: Drosophila, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified RNA polymerase II.
• Specificity: ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes.
  Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials.
  Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
  Cultured Cell Lines: Reacts with Drosophila chromosomes, PTK-2, HeLa, Hep2.
  Epitope Recognized: ⁷⁹⁴DDYGPESRGFVENSYLA⁸²².
• Formulation: State: Supernatant
  State: Culture Supernatant Concentrate
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: polymerase (RNA) II subunit A
• Database Link:
  Entrez Gene 5430 Human
  UniProt ID P24928
• Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
• Synonyms: RNA polymerase II, RNA polymerase II subunit B1, POLR2, POLRA, RPOL2, RPB1, RPB220

Reference Data

• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase