Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]
Other products for "VIM"
Specifications
Product Data | |
Clone Name | V9 |
Applications | IHC, WB |
Recommended Dilution | Western blotting. Immunohistochemistry on Frozen Sections: ~1/300 in PBS Immunohistochemistry on Formalin-Fixed, Paraffin Embedded Sections: ~1/50-1/100. Trypsin digestion of Paraffin Sections may be required. An excellent result on paraffin embedded tissues could be obtained with a TUF pretreatment. Positive Control: Sarcoma or Melanoma. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Vimentin isolated from Cow lens. |
Specificity | The antibody clone V9 stains the 57 kD Vimentin band in Immunoblots performed on a lysate of normal Human lymphocytes. It does not stain Mouse or Rat Vimentin. The antibody stains all cells containing Vimentin. Cross reactivity: GFAP: not detectable. Keratin : not detectable. Neurofilamen: not detectable. Desmin: not detectable. Cellular Localization: Cytoplasmic. |
Formulation | PBS State: Supernatant State: Liquid purified Supernatant Stabilizer: 1% BSA Preservative: 20 mM Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. |
Stability | Shelf life: One year from despatch. |
Gene Name | vimentin |
Database Link | |
Background | Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57 kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. |
Synonyms | VIM |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
{0} Product Review(s)
0 Product Review(s)
Submit review
Be the first one to submit a review
Product Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.