Histone H1 Mouse Monoclonal Antibody [Clone ID: AE-4]
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Specifications
Product Data | |
Clone Name | AE-4 |
Applications | ELISA, IF, IHC, IP, WB |
Recommended Dilution | ELISA. Western blotting. Immunoprecipitation. Immunofluorescence. Immunhistology on Frozen and Paraffin Sections: Acetone or PFA fixation is recommended for Frozen sections. Positive Control Tissue: Tonsil. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | A BALB/c mouse was immunized with Human leukemia biopsy cells. The isolated splenocytes were fused with mouse myeloma cells. |
Specificity | AE-4 recognizes Histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Tonsil is the recommended positive control tissue. |
Formulation | PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Background | The histone proteins serve as a structural scaffold for the organisation of nuclear DNA into chromatin. In eucariotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that asseble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. |
Synonyms | H1 Histone Family, H1F4, H1F5, Histone H1a, Histone H1b |
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