B-Cells (pan) Mouse Monoclonal Antibody [Clone ID: Ki-B1R]

CAT#: BM4013

B-Cells (pan) mouse monoclonal antibody, clone Ki-B1R, Purified

Product Images

Immunohistochemical staining on rat spleen frozen sections using B Lymphocytes antibody clone Ki-B1R Cat.-No. BM4013.

Specifications

Product Data

• Clone Name: Ki-B1R
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen sections: 1 µg/ml (1/100).
  Immunohistochemistry on Paraffin sections: 10 µg/ml (1/10). No antigen retrieval required, microwave treatment may enhance the reactivity.
  Suggested Positive Control: Rat spleen.
  Has been described to work in FACS.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat lymphocytes
• Specificity: Monoclonal Rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections.
  Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE.
  The antibody is specific for Rat mature B-lymphocytes and plasma cells. Other species not tested.
  Antigen Distribution:
  Isolated Cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% +/- 3% of peripheral blood lymphocytes (n=5).
  In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker.
  Tissue Sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo.
• Formulation: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative
  State: Purified
  State: Lyohilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.1 mg/ml (after reconstitution)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.
• Synonyms: B-cell marker, B cells