RET Chicken Polyclonal Antibody

CAT#: AP33454PU-N

RET chicken polyclonal antibody, Aff - Purified

Product Images

Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/80000-1/120000.
  Western blot: 1/5000-1/10000.
  Immunofluorescence: 1/250-1/500.
• Reactivities: Human
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based.
  The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant.
  Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
• Specificity: This antibody detects Human RET.
  Other species not tested.
• Formulation: Concentrated Ammonium Sulphate in PBS pH 7.4
  State: Aff - Purified
  State: Liquid purified IgY fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store at 2-8°C.
  As product is (NH4)₂SO₄ precipitate, mix well by pipetting or vortexing prior use.
• Stability: Shelf life: One year from despatch.
• Gene Name: ret proto-oncogene
• Database Link:
  Entrez Gene 5979 Human
  UniProt ID P07949
• Background: The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
• Synonyms: C-ret, CDHF12