TREX2 Goat Polyclonal Antibody

CAT#: AP31979PU-N

TREX2 goat polyclonal antibody, Aff - Purified

Product Images

TREX2 antibody staining of paraffin embedded Human Breast at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution:

  Peptide ELISA: 1/128000 (Detection limit).
  Western blot: Preliminary experiments gave an approx 18kDa band in lysates of Jurkat, MOLT4 and U937 after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.9kDa according to NP_542432.2. The 18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
  Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Human Breast shows nuclear staining in the luminal epithelial duct cells.

• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide with sequence from the internal region of Human TREX2 (NP_542432.2).
• Specificity: This antibody recognizes TREX2
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: three prime repair exonuclease 2
• Database Link:
  Entrez Gene 11219 Human
  UniProt ID Q9BQ50
• Background: TREX2 is a protein with 3' exonuclease activity. Enzymes with this activity are involved in DNA replication, repair, and recombination. Similarity to an E. coli protein suggests that this enzyme may be a subunit of DNA polymerase III, which does not have intrinsic exonuclease activity.
• Synonyms: OTTHUMP00000025920; OTTHUMP00000025922