CHMP4B Rabbit Polyclonal Antibody

CAT#: AP31897PU-N

CHMP4B rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  ELISA.
  Western Blot: 1/200-1/5000.
  Immunohistochemistry: 1/100-1/2000.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human CHMP4b protein.
• Specificity: Recognizes CHMP4b.
• Formulation: 0.1M Tris, 0.1M Glycine, 2% Sucrose
  State: Purified
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore in distilled water
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage:

  Lyophilized powder stable for a minimum of 2 years at -20°C.
  Store reconstituted antibody at 2-4°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: Six months from despatch.

• Gene Name: charged multivesicular body protein 4B
• Database Link:
  Entrez Gene 128866 Human
  UniProt ID Q9H444
• Background: The charged multivesicular body proteins or chromatin modifying proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), and are also required for the formation of these bodies. CHMP4B (charged multivesicular body protein 4b) is a 224 amino acid protein that interacts with Alix, which results in the redistribution of Alix from the cytoplasm to the perinuclear area. Overexpression of CHMP4B induces accumulation of ubiquinated proteins and inhibits degradation of endocytosed EGF. Defects in the gene encoding CHMP4B can cause posterior polar cataract type 3 (CTPP3), a disease characterized by distinct opacity located at the back of the lens resulting in visual impairment and eventual blindness.
• Synonyms: C20orf178, SHAX1, CHMP4b, SNF7-2, hSnf7-2, Vps32-2, hVps32-2