PTCH1 (C-term) Goat Polyclonal Antibody
PTCH1 (C-term) goat polyclonal antibody, Aff - Purified
Product Datasheet for 'AP22448PU-N'
|Applications||ELISA, IF, IHC|
|Recommend Dilution||Peptide ELISA: 1/8000 (Detection Limit).
Immunohsitochemistry on Paraffin Sections: 3-6 µg/ml.
Staining of Embedded Human Colon shows textured nuclear staining of the absorptive cells.
Immunofluorescence: 5 µg/ml. Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membrane of NIH3T3 cells.
|Reactivity||Human, Mouse, Rat|
|Immunogen||Peptide with sequence from the C Terminus of Human PTCH1 (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1)|
|Specificity||This antibody is expected to recognise all reported isoforms of PTCH1.
Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
|Formulation||Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide|
|Gene Name||Homo sapiens patched 1 (PTCH1), transcript variant 1b|
|Background||PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.
|Synonyms||PTCH, PTC1, PTC, Patched 1|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transmembrane|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer|
|The use of this Antibodies has been cited in the following citations:|
Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation.
,Gerardo Valadez J, Grover VK, Carter MD, Calcutt MW, Abiria SA, Lundberg CJ, Williams TV, Cooper MK,
Cancer Lett. 2018
,PubMed ID 23063752
Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling.
,Abiria SA, Williams TV, Munden AL, Grover VK, Wallace A, Lundberg CJ, Valadez JG, Cooper MK,
J. Neurooncol. 2014
,PubMed ID 24867209
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